Resistance Assessment for Oxathiapiprolin in Phytophthora capsici and the Detection of a Point Mutation (G769W) in PcORP1 that Confers Resistance.
Resistance Assessment for Oxathiapiprolin in Phytophthora capsici and the Detection of a Point Mutation (G769W) in PcORP1 that Confers Resistance.
复制标题
辣椒疫霉中 Oxathiapiprolin 的抗性评估以及 PcORP1 中赋予抗性的点突变 (G769W) 的检测
DOI：
10.3389/fmicb.2016.00615
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发表时间：
2016
影响因子：
5.2
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Miao J;Cai M;Dong X;Liu L;Lin D;Zhang C;Pang Z;Liu X
评估了辣椒疫霉对 oxathiapiprolin 抗性的潜力。最初确定了 175 个分离株对 oxathiapiprolin 的基线敏感性，发现符合平均 EC50 值为 5.61 × 10-4 μg/ml 的单峰曲线。通过在两个敏感分离株 LP3 和 HNJZ10 中进行杀菌剂适应，产生了 12 个稳定的 oxathiapiprolin 抗性突变体。发现 LP3 突变体的适应性与亲本分离株 LP3 相似或更好，而 HNJZ10 突变体被发现失去了产生游动孢子的能力。总而言之，这些结果表明辣椒疫病菌对 oxathiapiprolin 产生抗性的风险是中等的。对编码 oxathiapiprolin 靶蛋白的 LP3 突变体和野生型亲本分离株中的 PcORP1 基因进行比较，发现杂合突变导致氨基酸取代 G769W。突变的 PcORP1-769W 等位基因在敏感野生型分离株 BYA5 中的转化和表达证实，PcORP1 中的突变是观察到的 oxathiapiprolin 抗性的原因。最后开发了包括 As-PCR 和 CAP 在内的诊断测试，以检测辣椒疫病菌田间种群中 G769W 点突变引起的 oxathiapiprolin 抗性。
The potential for oxathiapiprolin resistance in Phytophthora capsici was evaluated. The baseline sensitivities of 175 isolates to oxathiapiprolin were initially determinated and found to conform to a unimodal curve with a mean EC50 value of 5.61 × 10-4 μg/ml. Twelve stable oxathiapiprolin-resistant mutants were generated by fungicide adaptation in two sensitive isolates, LP3 and HNJZ10. The fitness of the LP3-mutants was found to be similar to or better than that of the parental isolate LP3, while the HNJZ10-mutants were found to have lost the capacity to produce zoospores. Taken together these results suggest that the risk of P. capsici developing resistance to oxathiapiprolin is moderate. Comparison of the PcORP1 genes in the LP3-mutants and wild-type parental isolate, which encode the target protein of oxathiapiprolin, revealed that a heterozygous mutation caused the amino acid substitution G769W. Transformation and expression of the mutated PcORP1-769W allele in the sensitive wild-type isolate BYA5 confirmed that the mutation in PcORP1 was responsible for the observed oxathiapiprolin resistance. Finally diagnostic tests including As-PCR and CAPs were developed to detect the oxathiapiprolin resistance resulting from the G769W point mutation in field populations of P. capsici.