miR156a Mimic Represses the Epithelial-Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A.
miR156a Mimic Represses the Epithelial-Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A.
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miR156a Mimic 通过靶向连接粘附分子 A 抑制人鼻咽癌细胞的上皮-间质转化。
DOI：
10.1371/journal.pone.0157686
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Tian Y;Cai L;Tian Y;Tu Y;Qiu H;Xie G;Huang D;Zheng R;Zhang W
MicroRNAs（miRNAs）在癌症的发生发展中起着重要作用。西兰花是非常受欢迎的大群体的人口，并具有抗癌特性。连接粘附分子A（Junctional adhesion molecule A，JAMA）优先集中在紧密连接处，并影响细胞形态和迁移。上皮-间质转化（EMT）是与癌症进展和转移相关的发育程序。在这项研究中，我们的目的是研究西兰花的miRNA在人鼻咽癌（NPC）中的作用。我们通过测序技术在青花菜中发现了84个保守的miRNAs和184个新的miRNAs。其中miR 156 a表达最高。此外，合成的miR 156 a模拟物在体外抑制NPC细胞的EMT。此外，如通过3' UTR荧光素酶报告基因测定和蛋白质印迹所验证的，证实JAMA是miR 156 a模拟物的靶标。JAMA的下调与miR 156 a模拟物对NPC EMT的影响一致，JAMA的上调可部分恢复miR 156 a模拟物抑制的EMT。总之，这些结果表明miR 156 a模拟物通过靶向JAMA的3' UTR抑制NPC细胞的EMT。这些西兰花的miRNA谱为进一步研究提供了基础。此外，miR 156 a的发现可能对NPC患者的治疗具有临床意义。
MicroRNAs (miRNAs) have been documented as having an important role in the development of cancer. Broccoli is very popular in large groups of the population and has anticancer properties. Junctional adhesion molecule A (JAMA) is preferentially concentrated at tight junctions and influences cell morphology and migration. Epithelial–mesenchymal transition (EMT) is a developmental program associated with cancer progression and metastasis. In this study we aimed to investigate the role of miRNAs from broccoli in human nasopharyngeal cancer (NPC). We demonstrated that a total of 84 conserved miRNAs and 184 putative novel miRNAs were found in broccoli by sequencing technology. Among these, miR156a was expressed the most. In addition, synthetic miR156a mimic inhibited the EMT of NPC cells in vitro. Furthermore, it was confirmed that JAMA was the target of miR156a mimic as validated by 3’ UTR luciferase reporter assays and western blotting. Knockdown of JAMA was consistent with the effects of miR156a mimic on the EMT of NPC, and the up-regulation of JAMA could partially restore EMT repressed by miR156a mimic. In conclusion, these results indicate that the miR156a mimic inhibits the EMT of NPC cells by targeting the 3’ UTR of JAMA. These miRNA profiles of broccoli provide a fundamental basis for further research. Moreover, the discovery of miR156a may have clinical implications for the treatment of patients with NPC.