An in vivo preparation for investigating neural responses to controlled loading of a lumbar vertebra in the anesthetized cat.
An in vivo preparation for investigating neural responses to controlled loading of a lumbar vertebra in the anesthetized cat.
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一种体内制剂，用于研究麻醉猫腰椎受控负荷的神经反应。
DOI：
10.1016/s0165-0270(99)00060-6
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发表时间：
1999
影响因子：
3
通讯作者：
Pickar,JG
中科院分区：
文献类型：
--
作者：
Pickar,JG
本文描述了一种向腰椎施加受控载荷的方法，同时在体内记录神经支配腰部脊柱旁组织的初级传入神经。与无脊椎骨骼不同，脊柱对神经生理学研究提出了独特的挑战。椎旁组织和脊髓之间的距离很短。此外，大量切除椎旁组织以接近脊神经根或脊髓似乎是必要的。本报告所述的准备利用了L 6背根通过椎间孔进入脊髓头侧2-2.5个椎体节段的解剖学事实。这有效地延长了腰椎椎旁组织和中央记录部位之间的距离。该制备物具有五个独特的特征：（1）L 6和L7椎骨保持完整;（2）腰椎椎旁组织及其与L 6和L7椎骨的附着在脊柱的一侧保持完整;（3）完整的L 6椎骨可在其棘突处加载;（4）施加在L 6棘突处的载荷的大小可由反馈马达控制;（5）L 6棘突处的载荷的大小可由反馈马达控制。（5）可以控制载荷相对于脊柱长轴的方向。使用这种制备方法，在L 6腰椎棘突处受控加载期间，从L 6背根获得单个单位记录。两个椎旁肌本体感受器椎体负荷的反应的特点是在这份报告中。与现有的电生理技术，这种准备可用于研究脊髓旁输入的中央处理。通过在炎症介质存在下结合腰椎的机械负荷，这种制备可以有助于理解机械和化学刺激之间的相互作用可能产生腰痛的机制。
This paper describes a method for applying controlled loads to a lumbar vertebra while recording in vivo from primary afferents innervating the lumbar paraspinal tissues. Unlike the appendicular skeleton, the vertebral column poses a unique challenge for neurophysiological investigations. Distances between paraspinal tissues and the spinal cord are short. In addition, substantial removal of the paraspinal tissues to access the spinal roots or spinal cord appears necessary. The preparation described in this report takes advantage of the anatomical fact that the L6dorsal root enters the spinal cord 2–2.5 vertebral segments rostral to its passage through the intervertebral foramina. This effectively lengthens the distance between the lumbar paraspinal tissues and central recording sites. The preparation has five unique features: (1) the L6and L7vertebrae remain intact; (2) lumbar paraspinal tissues and their attachments to the L6and L7vertebrae remain intact on one side of the vertebral column; (3) the intact L6vertebra can be loaded at its spinous process; (4) the magnitude of the load applied at the L6spinous process can be controlled with a feedback motor; (5) the direction of load relative to the long axis of the spine can be controlled. Using this preparation, single unit recordings were obtained from the L6dorsal root during controlled loading of the L6lumbar vertebra at its spinous process. The responses of two paraspinal muscle proprioceptors to vertebral loading are characterized in this report. With existing electrophysiological techniques this preparation can be used to study central processing of paraspinal inputs. By combining mechanical loading of the lumbar spine in the presence of inflammatory mediators this preparation can contribute to the understanding of the mechanisms by which interactions between mechanical and chemical stimuli likely produce low back pain.