Expression of somatostatin and somatostatin receptor subtypes 1-5 in human normal and diseased kidney
Expression of somatostatin and somatostatin receptor subtypes 1-5 in human normal and diseased kidney
复制标题
DOI：
10.1369/jhc.2008.950998
复制
发表时间：
2008-08-01
影响因子：
3.2
通讯作者：
Atkin, Stephen L.
中科院分区：
文献类型：
--
作者：
Bhandari, Sunil;Watson, Neil;Atkin, Stephen L.
生长抑素通过5个G蛋白偶联的生长抑素受体（sst（1-5））介导抑制功能。我们用免疫组织化学、免疫荧光和RT-PCR方法检测了正常人和伊加肾病患者肾脏中生长抑素受体sst（1）、sst（2A）、sst（2B）、sst（3）、sst（4）和sst（5）的存在。所有生长抑素受体在肾切除术和活检标本的组织切片中的细小管（二曲小管和Henle袢）和粗小管（近曲小管）中检测到。SST（1）、SST（4）免疫阳性染色多集中于肾小管上皮细胞胞浆。在正常肾组织切片中，肾小球中的足细胞和系膜细胞对sst（1）、sst（2B）、sst（4）和sst（5）染色，对sst（3）染色较弱。在伊加肾组织中，生长抑素受体的表达显著增加，肾小球内sst（1）、sst（2B）、sst（4）和sst（5）免疫阳性染色。在上皮细胞中，近端小管中的sst（2B）和sst（4）以及远端小管中的sst（1）、sst（2B）和sst（5）染色增加。RT-PCR检测sst（1-5）mRNA的表达。生长抑素及其受体亚型在正常肾脏和伊加肾病中广泛分布。伊加肾病肾组织生长抑素受体表达增加可能是炎症性肾病的潜在发病机制。
Somatostatin mediates inhibitory functions through five G protein-coupled somatostatin receptors (sst(1-5)). We used immunohistochemistry, immunofluorescence, and RT-PCR to determine the presence of somatostatin receptors sst(1), sst(2A), sst(2B), sst(3), sst(4), and sst(5) in normal and IgA nephropathy human kidney. All somatostatin receptors were detected in the thin tubules (di5tal convoluted tubules and loops of Henle) and thick tubules (proximal convoluted tubules) in the tissue sections from nephrectomy and biopsy samples. Immunopositive sst(1) and sst(4) staining was more condensed in the cytoplasm of tubular epithelial cells. In normal kidney tissue sections, podocytes and mesangial cells in the glomeruli stained for sst(1), sst(2B), sst(4) and sst(5), and stained weakly for sst(3). In IgA kidney tissue, the expression of somatostatin receptors was significantly increased with particular immmuno-positive staining for sst(1), sst(2B), sst(4), and sst(5) within glomeruli. In the epithelial cells, the staining for sst(2B) and sst(4) in proximal tubules and sst(1), sst(2B), and sst(5) in distal tubules was increased. The mRNA expression of sst(1-5) was also detected by RT-PCR. Somatostatin and all five receptor subtypes were ubiquitously distributed in normal kidney and IgA nephropathy. The increased expression of somatostatin receptors in IgA nephropathy kidney might be the potential pathogenesis of inflammatory renal disease.