Induction of an interferon response by RNAi vectors in mammalian cells
Induction of an interferon response by RNAi vectors in mammalian cells
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DOI：
10.1038/ng1173
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发表时间：
2003-07-01
期刊：
影响因子：
30.8
通讯作者：
Iggo, R
中科院分区：
文献类型：
--
作者：
Bridge, AJ;Pebernard, S;Iggo, R
从RNA聚合酶III（Pol III）启动子表达短发夹RNA（shRNA）的DNA载体是减少哺乳动物细胞中基因表达的有前途的新工具。shRNA被加工成21个核苷酸（nt）的小干扰RNA（siRNA），其通过RNA诱导的沉默复合物引导同源mRNA的切割。虽然siRNA被认为太短而不能诱导干扰素表达，但我们在此报告了大量的shRNA载体可以触发干扰素应答。
DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.