Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface
Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface
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DOI：
10.1126/science.1079546
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发表时间：
2003-01-17
期刊：
影响因子：
56.9
通讯作者：
Fisher, SJ
中科院分区：
文献类型：
--
作者：
Genbacev, OD;Prakobphol, A;Fisher, SJ
滋养层粘附在子宫壁上是着床和随后胎盘形成的必要的第一步。在母胎界面，我们研究了选择素粘附系统的表达，使白细胞从血流中捕获。在母体侧，人子宫上皮细胞在接受性窗口期间上调选择素寡糖基配体。在胎儿侧，人滋养层细胞表达L-选择素。这种配体-受体系统是功能性的，因为包被有选择素配体6-磺基sLe（x）的珠与滋养层结合，而滋养层与组织切片中表达配体的子宫腔上皮结合。这些结果表明，滋养层L-选择素介导与子宫的相互作用，这种粘附机制可能是建立人类妊娠的关键。
Trophoblast adhesion to the uterine wall is the requisite first step of implantation and, subsequently, placentation. At the maternal-fetal interface, we investigated the expression of selectin adhesion systems that enable leukocyte capture from the bloodstream. On the maternal side, human uterine epithelial cells up-regulated selectin oligosaccharide-based ligands during the window of receptivity. On the fetal side, human trophoblasts expressed L-selectin. This ligand-receptor system was functional, because beads coated with the selectin ligand 6-sulfo sLe(x) bound to trophoblasts, and trophoblasts bound to ligand-expressing uterine luminal epithelium in tissue sections. These results suggest that trophoblast L-selectin mediates interactions with the uterus and that this adhesion mechanism may be critical to establishing human pregnancy.