Cell-imprinted polydimethylsiloxane for the selective cell adhesion
Cell-imprinted polydimethylsiloxane for the selective cell adhesion
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用于选择性细胞粘附的细胞印迹聚二甲基硅氧烷
DOI：
10.1016/j.cclet.2018.11.006
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发表时间：
2019
影响因子：
9.1
通讯作者：
Zhang Yukui
中科院分区：
文献类型：
--
作者：
Liu Lukuan;Yang Kaiguang;Dai Zhongpeng;Liang Zhen;Zhang Lihua;Peng Xiaojun;Zhang Yukui
细胞粘附是某些细胞分离方法的基础，并受到基质的生化和形貌特征的影响。在此，基于细胞印迹和点击化学，我们开发了一种具有适体功能化的细胞印迹聚二甲基硅氧烷（PDMS）（APT-CIS）。原子力显微镜分析结果表明，与靶细胞匹配良好的分级结构成功引入到APT-CIS表面。利用分层结构和适配体亲和力的协同作用，APT-CIS成功地用于选择性细胞粘附，并且可以释放93.9%±0.8%的捕获细胞。因此，APT-CIS 在选择性细胞分离和分选领域具有广阔的前景。
Cell adhesion is the basis for some cell isolation methods, and is influenced by both of the biochemical and topographic characteristics of the substrates. Herein, based on cell imprinting and click chemistry, we have developed a cell-imprinted polydimethylsiloxane (PDMS) with aptamer functionalization (APT-CIS). The atom force microscopic analysis results showed that the hierarchical structure matching well with the target cells is successfully introduced on the surface of the APT-CIS. By using the synergistic effects of hierarchical structure and aptamer affinity, the APT-CIS was successfully used for the selective cell adhesion, and 93.9% ± 0.8% of the captured cells could then be released. Thus, the APT-CIS holds promise in selective cell isolation and sorting fields.