Alleviation effects of Bifidobacterium breve on DSS-induced colitis depends on intestinal tract barrier maintenance and gut microbiota modulation
Alleviation effects of Bifidobacterium breve on DSS-induced colitis depends on intestinal tract barrier maintenance and gut microbiota modulation
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短双歧杆菌对 DSS 诱导的结肠炎的缓解作用取决于肠道屏障的维护和肠道微生物群的调节
DOI：
10.1007/s00394-020-02252-x
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发表时间：
2020-04-29
影响因子：
5
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Chen, Yang;Jin, Yan;Chen, Wei
目的：探讨不同产CLA短双歧杆菌对葡聚糖硫酸钠（DSS）诱导的结肠炎的差异及潜在机制。方法：采用DSS诱导C57BL/6 J小鼠结肠炎。测定疾病活动指数（DAI）、组织病理学变化、上皮屏障完整性和上皮细胞凋亡。通过测定肠道菌群来评估产CLA的全身效应。结果：口服不同的短双歧杆菌表现出不同的效果，其中短双歧杆菌M1和短双歧杆菌M2减轻了DSS诱导的炎症，并显着增加了粘蛋白2（MUC2）和杯状细胞的浓度，但短双歧杆菌M3和短双歧杆菌M4均没有这种保护作用。同时，短双歧杆菌 M1 和短双歧杆菌 M2 处理显着上调紧密连接 (TJ) 蛋白，并改善 DSS 攻击导致的上皮细胞凋亡。此外，炎症细胞因子（TNF-α、IL-6）受短双歧杆菌 M1 和短双歧杆菌 M2 调节，而短双歧杆菌 M3 和短双歧杆菌 M4 均不调节。此外，B. breve M1 和 B. breve M2 降低了拟杆菌的丰度，增加了臭杆菌的丰度，然后重新平衡了受损的肠道微生物群。饲喂短双歧杆菌M1、短双歧杆菌M2、短双歧杆菌M3和短双歧杆菌M4的小鼠结肠中CLA浓度依次降低，与缓解结肠炎的效果呈显着正相关。结论：短双歧杆菌M1和短双歧杆菌M2通过产生CLA、抑制炎症细胞因子、维持肠上皮屏障和调节肠道菌群，缓解DSS诱导的结肠炎。
Purpose:The study aimed to investigate the discrepancy and potential mechanisms of different CLA-producing B. breve on dextran sulphate sodium (DSS)-induced colitis.Methods:Colitis was induced in C57BL/6 J mice using DSS. Disease activity index (DAI), histopathological changes, epithelial barrier integrity and epithelial apoptosis were determined. Gut microbiota were gauged to evaluate the systemic effects of CLA-producing B. breve.Results:Oral administration of different B. breve showed different effects, in which B. breve M1 and B. breve M2 alleviated the inflammation induced by DSS as well as significantly increased the concentration of mucin2 (MUC2) and goblet cells, but neither B. breve M3 nor B. breve M4 had those protective effects. Meanwhile, B. breve M1 and B. breve M2 treatments significantly up-regulated the tight junction (TJ) proteins and ameliorated the epithelial apoptosis lead by DSS challenge. Moreover, inflammatory cytokines (TNF-α, IL-6) were modulated by B. breve M1 and B. breve M2, neither B. breve M3 nor B. breve M4. Furthermore, B. breve M1 and B. breve M2 reduced the abundance of Bacteroides and increased the abundance of Odoribacter, then rebalanced the damaged gut microbiota. Colonic CLA concentrations in mice fed with B. breve M1, B. breve M2, B. breve M3 and B. breve M4 decreased successively, which showed significant positive correlation with the effectiveness of relieving colitis.Conclusions:Bifidobacterium breve M1 and B. breve M2 alleviated DSS-induced colitis by producing CLA, inhibiting the inflammatory cytokines, maintaining of the intestinal epithelial barrier and regulating the gut microbiota.