Flavobacterium psychrophilum vaccine development: a difficult task.
Flavobacterium psychrophilum vaccine development: a difficult task.
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基质疫苗的开发：一项艰巨的任务。
DOI：
10.1111/1751-7915.12099
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发表时间：
2014-09
影响因子：
5.7
通讯作者：
Guijarro JA
中科院分区：
文献类型：
--
作者：
Gómez E;Méndez J;Cascales D;Guijarro JA
细菌性冷水病（BCWD）是由革兰氏阴性细菌嗜冷黄杆菌（Flavobacterium psychrophilum）引起的一种全球性淡水鱼疾病。这是一个特别毁灭性的感染鱼苗鲑科鱼类，并可能导致高水平的死亡率。尽管它对养鱼场的经济影响，但细菌的生物学和细菌与宿主的相互作用都没有得到很好的理解。这篇综述提供了一个概要的主要问题有关的关键剩余问题的研究使用疫苗对F。psychrophilum和针对这种疾病的商业疫苗的开发。使用来自恢复期虹鳟鱼的血清的研究已经显示了不同蛋白质如OmpH、OmpA和FspA以及F.嗜冷菌，这是潜在的候选亚单位疫苗。灭活F.在实验室条件下，通过浸渍和腹膜内途径，嗜冷菌蛋白已成功地作为疫苗进行了测试。然而，这些制剂的有效性和实用性仍有待证明。使用减毒和野生型菌株免疫鱼类表明，这些系统提供了高水平的保护。然而，它们的适用与许多国家的环境保护法规相冲突。总之，针对BCWD的保护性疫苗在理论上是可能的，但是为了允许商业疫苗的开发，仍然必须进行大量的努力。
Bacterial cold water disease (BCWD) is a globally distributed freshwater fish disease caused by the Gram-negative bacterium Flavobacterium psychrophilum. It is a particularly devastating infection in fry salmonids and may lead to high levels of mortality. In spite of its economic impact on fish farms, neither the biology of the bacterium nor the bacterium–host interactions are well understood. This review provides a synopsis of the major problems related to critical remaining questions about research into the use of vaccines against F. psychrophilum and the development of a commercial vaccine against this disease. Studies using sera from convalescent rainbow trout have shown the antigenic properties of different proteins such as OmpH, OmpA and FspA, as well as low and high molecular mass lipopolysaccharide of F. psychrophilum, which are potential candidates for subunit vaccines. Inactivated F. psychrophilum bacterins have been successfully tested as vaccines under laboratory conditions by both immersion and intraperitoneal routes. However, the efficacy and the practical usefulness of these preparations still have to be proved. The use of attenuated and wild-type strains to immunize fish showed that these systems offer high levels of protection. Nevertheless, their application clashes with the regulations for environmental protection in many countries. In conclusion, protective vaccines against BCWD are theoretically possible, but substantial efforts still have to be made in order to permit the development of a commercial vaccine.
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影响因子：
4
作者：
Dumetz, F.;LaPatra, S.-E.;Le Henaff, M.
通讯作者：
Le Henaff, M.
影响因子：
4.4
作者：
Alvarez, B.;Secades, P.;Guijarro, J. A.
通讯作者：
Guijarro, J. A.
影响因子：
4.4
作者：
Dumetz, Fabien;Duchaud, Eric;Le Henaff, Michel
通讯作者：
Le Henaff, Michel
影响因子：
1.4
作者：
Brown, LL;Cox, WT;Levine, RP
通讯作者：
Levine, RP
影响因子：
2.8
作者：
Alvarez, Beatriz;Alvarez, Javier;Guijarro, Jose A.
通讯作者：
Guijarro, Jose A.