Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020
Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020
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DOI：
10.2807/1560-7917.es.2020.25.24.2001057
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发表时间：
2020-06-18
期刊：
影响因子：
19
通讯作者：
Keppler, Oliver T.
中科院分区：
文献类型：
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作者：
Muenchhoff, Maximilian;Mairhofer, Helga;Keppler, Oliver T.
当前大流行期间冠状病毒病 (COVID-19) 患者的遏制策略和临床管理取决于对严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的可靠诊断 PCR 检测。在这里，我们比较了 2020 年 3 月德国七个诊断实验室使用的 11 种不同的 RT-PCR 测试系统。虽然大多数检测方法表现良好，但我们发现了常用检测方法中的检测问题，这些问题可能导致大流行第一周出现假阴性检测结果。
Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.