PARTICIPATION OF PEROXISOMES IN LIPID BIOSYNTHESIS IN THE HARDERIAN-GLAND OF GUINEA-PIG
PARTICIPATION OF PEROXISOMES IN LIPID BIOSYNTHESIS IN THE HARDERIAN-GLAND OF GUINEA-PIG
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DOI：
10.1042/bj2620677
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发表时间：
1989-09-01
影响因子：
4.1
通讯作者：
SUGA, T
中科院分区：
文献类型：
--
作者：
HORIE, S;SUGA, T
研究了具有独特脂质组成的豚鼠哈氏腺中过氧化物酶体的活性。过氧化氢酶、酰基辅酶A氧化酶和氰化物不敏感的酰基辅酶A β-氧化系统与大鼠肝脏的氧化系统相似。磷酸二羟丙酮酰基转移酶（DHAPAT，EC 2.3.1.42）和烷基-DHAP合酶（EC 2.5.1.26）活性较高，且活性分布与可沉淀过氧化氢酶活性分布一致。甘油-3-磷酸酰基转移酶（GPAT，EC 2.3.1.15）位于大鼠肝脏的微粒体（微粒体部分）和线粒体中，是哈氏腺中的过氧化物酶体酶，尽管其活性仅为DHAPAT活性的约十分之一。这些酶具有不同的pH曲线和底物特异性。过氧化物酶体中存在高活性的酰基-DHAP途径的酶，提示过氧化物酶体在豚鼠哈氏腺甘油醚磷脂和1-烷基-2，3-二酰基甘油的生物合成中具有重要的生理意义。
Peroxisomal enzyme activities in the guinea-pig harderian gland, which has a unique lipid composition, were studied. Activities of catalase, acyl-CoA oxidase and the cyanide-insensitive acyl-CoA .beta.-oxidation system in this tissue were comparable with those in rat liver. The activities of dihydroxyacetone phosphate acyltransferase (DHAPAT, EC 2.3.1.42) and alkyl-DHAP synthase (EC 2.5.1.26) were appreciable, and the distributions of both activities were consistent with that of sedimentable catalase activity. Glycerol-3-phosphate acyltransferase (GPAT, EC 2.3.1.15), which is localized in both microsomes (microsomal fractions) and mitochondria in the rat liver, was a peroxisomal enzyme in the harderian gland, though the activity was only about one-tenth of the DHAPAT activity. These enzymes had different pH profiles and substrate specificity. The existence of high activites of enzymes of the acyl-DHAP pathway in peroxisomes suggests the physiological significance of peroxisomes in the biosynthesis of glycerol ether phospholipid and 1-alkyl-2,3-diacylglycerol in the guinea-pig harderian gland.