Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum.
Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum.
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DOI：
10.17113/ftb.52.04.14.3701
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发表时间：
2014-12
影响因子：
2.4
通讯作者：
M. Melloul;D. Iraqi;MyAbdelaziz El Alaoui;G. Erba;S. Alaoui;M. Ibriz;E. Elfahime
中科院分区：
文献类型：
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作者：
M. Melloul;D. Iraqi;MyAbdelaziz El Alaoui;G. Erba;S. Alaoui;M. Ibriz;E. Elfahime
干旱是导致作物减产的最大的单一非生物胁迫因素。鉴定差异表达基因并了解其在环境胁迫条件下的功能对于提高耐旱性至关重要。转录组学是一种强有力的方法，在非生物胁迫下的分子机制的整体分析。为了鉴定耐旱性的重要基因，我们用cDNA扩增片段长度多态性（cDNA-AFLP）技术分析了干旱胁迫和未处理的硬粒小麦叶片的mRNA群体。总的来说，76个转录衍生片段对应于差异诱导转录成功测序。利用基本的局部比对搜索工具（BLAST）数据库，在这里鉴定的大部分转录本是属于与代谢、能量、细胞生物合成、细胞防御、信号转导、转录调节、蛋白质降解和运输相关的不同功能类别的基因。这些基因的表达模式通过定量逆转录酶实时聚合酶链反应（qRT-PCR）基于代表不同模式的10个选择的基因来确认。这些结果可以促进理解的细胞机制，涉及组的基因，在响应水分亏缺的刺激协调行动。新的胁迫响应基因的鉴定将提供有用的数据，可以帮助开发旨在提高硬粒小麦田间胁迫耐受性的育种策略。
Drought is the single largest abiotic stress factor leading to reduced crop yields. The identification of differentially expressed genes and the understanding of their functions in environmentally stressful conditions are essential to improve drought tolerance. Transcriptomics is a powerful approach for the global analysis of molecular mechanisms under abiotic stress. To identify genes that are important for drought tolerance, we analyzed mRNA populations from untreated and drought-stressed leaves of Triticum durum by cDNA- -amplified fragment length polymorphism (cDNA-AFLP) technique. Overall, 76 transcript- -derived fragments corresponding to differentially induced transcripts were successfully sequenced. Most of the transcripts identified here, using basic local alignment search tool (BLAST) database, were genes belonging to different functional categories related to metabolism, energy, cellular biosynthesis, cell defense, signal transduction, transcription regulation, protein degradation and transport. The expression patterns of these genes were confirmed by quantitative reverse transcriptase real-time polymerase chain reaction (qRT- -PCR) based on ten selected genes representing different patterns. These results could facilitate the understanding of cellular mechanisms involving groups of genes that act in coordination in response to stimuli of water deficit. The identification of novel stress-responsive genes will provide useful data that could help develop breeding strategies aimed at improving durum wheat tolerance to field stress.