Influence of Light Intensity on Growth and Pigment Production by Monascus ruber in Submerged Fermentation
Influence of Light Intensity on Growth and Pigment Production by Monascus ruber in Submerged Fermentation
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DOI：
10.1007/s12010-015-1645-8
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发表时间：
2015-07-01
影响因子：
3
通讯作者：
Ninow, Jorge Luiz
中科院分区：
文献类型：
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作者：
Meinicke Buehler, Rose Marie;Mueler, Bruna Luise;Ninow, Jorge Luiz
为了减少甘油积累造成的环境问题，并使生物柴油的生产更有利可图，未经处理的粗甘油被用作底物，以获得高附加值的生物产品。红曲霉是一种丝状真菌，能产生色素，尤其是红色色素，用于给食品（米酒和肉制品）着色。由于使用合成染料的限制，从天然来源开发颜料的兴趣正在增加。研究了温度、pH、微生物形态、曝气、氮源和基质对橡胶乳杆菌培养的影响。在这项工作中，我们观察到光强度也是了解真菌代谢的一个重要因素。在橡胶分枝杆菌的培养中，在直接光照下的培养皿和蒙面烧瓶中观察到生长和色素产生的抑制。培养皿和烧瓶暴露在红光下和没有光的情况下，生长和色素产量更高。在黑暗条件下，橡胶分枝杆菌的径向生长速率为1.50 mm day(-1)，在直接光照条件下，径向生长速率为0.59 mm day（-1）。在覆盖红膜的折板烧瓶中，红色素和生物量的产量最高，分别为8.32 UA和8.82 g L-1，红色素的产量为7.17 UA，在黑暗中培养的烧瓶中，生物量的产量为7.40 g L-1。在1248 lux的光照条件下，该真菌的最大色素产量为4.48 UA，生物量产量为6.94 g L-1，表明该真菌具有影响生理反应的光感受器。
To reduce environmental problems caused by glycerine accumulation and to make the production of biodiesel more profitable, crude glycerin without treatment was used as substrate for obtaining higher value-added bioproducts. Monascus ruber is a filamentous fungus that produces pigments, particularly red ones, which are used for coloring foods (rice wine and meat products). The interest in developing pigments from natural sources is increasing due to the restriction of using synthetic dyes. The effects of temperature, pH, microorganism morphology, aeration, nitrogen source, and substrates have been studied in the cultivation of M. ruber. In this work, it was observed that light intensity is also an important factor that should be considered for understanding the metabolism of the fungus. In M. ruber cultivation, inhibition of growth and pigment production was observed in Petri dishes and blaffed flasks exposed to direct illumination. Growth and pigment production were higher in Petri dishes and flasks exposed to red light and in the absence of light. Radial growth rate of M. ruber in plates in darkness was 1.50 mm day(-1) and in plates exposed to direct illumination was 0.59 mm day(-1). Maximum production of red pigments (8.32 UA) and biomass (8.82 g L-1) were obtained in baffled flasks covered with red film and 7.17 UA of red pigments, and 7.40 g L-1 of biomass was obtained in flasks incubated in darkness. Under conditions of 1248 lux of luminance, the maximum pigment production was 4.48 UA, with production of 6.94 g L-1 of biomass, indicating that the fungus has photoreceptors which influence the physiological responses.