High load of Merkel cell polyomavirus DNA detected in the normal skin of Japanese patients with Merkel cell carcinoma
High load of Merkel cell polyomavirus DNA detected in the normal skin of Japanese patients with Merkel cell carcinoma
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DOI：
10.1016/j.jcv.2016.07.011
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发表时间：
2016-09-01
影响因子：
8.8
通讯作者：
Daibata, Masanori
中科院分区：
文献类型：
--
作者：
Hashida, Yumiko;Nakajima, Kimiko;Daibata, Masanori
背景资料：虽然默克尔细胞多瘤病毒（MCPyV）具有引起默克尔细胞癌（MCC）的潜力，但它也存在于健康个体的正常皮肤中。然而，MCPyV转化为致癌形式的机制是unknown.Objectives：调查MCPyV感染的水平在正常皮肤患者MCC与对照coherent.Study设计：我们研究了总共6个日本患者皮肤MCC。太阳暴露和太阳未暴露的皮肤拭子，获得和分析MCPyV负载使用定量实时polymerase chain reaction.Results：首先，我们发现了一个病人MCC携带极高负荷的MCPyV DNA在正常皮肤。这一独特的病例促使我们进一步探索MCC患者皮肤微生物群中MCPyV的水平。我们发现，MCPyV DNA水平显着较高的拭子从正常皮肤样本的6例MCC相比，从30个年龄匹配的健康人和19例其他皮肤癌患者。然而，从MCC患者的正常皮肤获得的MCPyV毒株具有没有结构改变的基因序列，而肿瘤衍生的毒株的序列显示截短突变或缺失。虽然研究的MCC患者数量很少，但我们的研究结果表明，MCC可能在皮肤中存在高MCPyV载量的背景下发生，并有望刺激进一步研究这种皮肤病毒组水平是否可以作为MCC发展的预测标志物之一。(C)© 2016 Elsevier B.V.版权所有。
Background: Although Merkel cell polyomavirus (MCPyV) has the potential to cause Merkel cell carcinoma (MCC), it is also found in the normal skin of healthy individuals. However, the mechanism for transformation of MCPyV to an oncogenic form is unknown.Objectives: To investigate the levels of MCPyV infection in the normal skin patients with MCC compared with those in a control cohort.Study design: We studied a total of six Japanese patients with cutaneous MCC. Sun-exposed and sun unexposed skin swabs were obtained and analyzed for MCPyV loads using quantitative real-time polymerase chain reaction.Results: At first, we found a patient with MCC carrying an extremely high load of MCPyV DNA in normal skin. This unique case prompted us to further explore the levels of MCPyV as skin microbiota in patients with MCC. We showed that MCPyV DNA levels were significantly higher in swabs obtained from normal skin samples of six patients with MCC compared with those from 30 age-matched healthy individuals and 19 patients with other cutaneous cancers. Whereas MCPyV strains obtained from the normal skin of patients with MCC had gene sequences without structural alterations, sequences of the tumor-derived strains showed truncating mutations or deletions.Conclusions: Although the number of patients with MCC studied was small, our findings suggest that MCC may occur with a background of high MCPyV load in the skin, and are expected to stimulate further studies on whether such skin virome levels could be one of predictive markers for the development of MCC. (C) 2016 Elsevier B.V. All rights reserved.