Nano-gold plasmon coupled with dual-function quercetin for enhanced photoelectrochemical aptasensor of tetracycline
Nano-gold plasmon coupled with dual-function quercetin for enhanced photoelectrochemical aptasensor of tetracycline
复制标题
纳米金等离子激元与双功能槲皮素偶联增强四环素光电化学适配体传感器
DOI：
10.1016/j.snb.2016.12.032
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Wei Wang
中科院分区：
文献类型：
--
作者：
Hongbo Li;Jing Li;Yunfei Qiao;Hailin Fang;Dahe Fan;Wei Wang
利用纳米金和双功能槲皮素的局域表面等离子体共振（LSPR）增强光电流响应策略，构建了一种简单、新颖的四环素（Tc）光电化学适体传感器。在模拟光的激发下，纳米Au/TiO 2比纯TiO 2纳米粒子的光电流增加，这是由于受激Au纳米粒子产生的超热电子所致。此外，选择槲皮素作为光敏剂和电子牺牲剂，以扩大光电流，从而提高PEC适体传感器的灵敏度。优异的选择性归因于Tc与其适体的特异性结合。在优化的条件下，PEC响应与Tc的浓度在0.3至1600 nmol L-1范围内呈线性关系，检测限为0.1 nmol L-1。其它常规有机物对测定无干扰，回收率在93.7%~ 105.3%之间。最后，将该传感器应用于液态奶样品中Tc的检测，结果表明，该传感器具有线性范围宽、成本低、选择性好、灵敏度高等优点。此外，它提供了一种检测Tc的替代方法。
A simple and novel photoelectrochemical (PEC) aptasensor for tetracycline (Tc) was developed using the enhanced photocurrent response strategy by localized surface plasmon resonance (LSPR) of nano-Au and dual-function quercetin. With the photoexcitation of simulated light, the increased photocurrent for nano-Au/TiO2than that of pure TiO2nanoparticles is attributed to the hot electrons from excited Au nanoparticles. Also, quercetin was chosen as both photosensitizer and electron sacrificial agent for the enlarged photocurrents and thus the high sensitivity of the PEC aptasensor. The excellent selectivity is assigned to the specific binding of Tc to its aptamer. On the optimized condition, the PEC response was linear with the concentration of Tc in the range from 0.3 to 1600 nmol L−1with a detection limit of 0.1 nmol L−1. The same concentration of other conventional organics did not interfere in the detection of Tc and the recovery was between 93.7% and 105.3%. Finally, the PEC aptasensor was successfully for the detection of Tc in liquid milk samples with good performance in wide linear range, low cost, high selectivity and sensitivity. Also, it provided an alternative approach to the detection of Tc.