Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae
Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae
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DOI：
10.1007/s10681-011-0393-2
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发表时间：
2011-09-01
期刊：
影响因子：
1.9
通讯作者：
Nakano, Masaru
中科院分区：
文献类型：
--
作者：
Nakazawa, Daisuke;Kishimoto, Tomonari;Nakano, Masaru
一些属间杂交种是由几个秋水仙系观赏植物杂交产生的，Gloriosa superba 'Lutea' (2n = 2x = 22)， G。‘Marron Gold’ （2n = 4x = 44）， G。‘Verschild’ （2n = 7x = 77）， Littonia modesa Hook。（2n = 2x = 22）；（2n = 2x = 24），进行基因组原位杂交（GISH）分析，以明确其基因组构成。以鸢尾草×超级鸢尾’、鸢尾草×金缕草、金缕草×超级鸢尾’、鸢尾草×金缕草的根尖细胞为材料进行染色体制备。“Marron Gold”，S. aurantiaca x G。“Marron Gold”和G。‘Verschild’ x S. aurantiaca。其中一个亲本用地高辛或生物素标记总DNA作为探针，染色体用4′-6-二胺酮-2-苯基吲哚（DAPI）反染。所有9个属间杂交种的染色体均可通过GISH分析清楚地区分出亲本的染色体。因此，GISH分析是鉴定秋水仙属观赏植物属间杂种基因组构成的有力工具。GISH分析研究结果对进一步开展秋水仙属观赏植物育种具有重要意义。
Some intergeneric hybrids produced by the crosses among several colchicaceous ornamentals, Gloriosa superba 'Lutea' (2n = 2x = 22), G. 'Marron Gold' (2n = 4x = 44), G. 'Verschild' (2n = 7x = 77), Littonia modesta Hook. (2n = 2x = 22), and Sandersonia aurantiaca Hook. (2n = 2x = 24), were subjected to genomic in situ hybridization (GISH) analysis in order to clarify their genome constitutions. Chromosome preparation was made from root tip cells of L. modesta x G. superba 'Lutea', L. modesta x S. aurantiaca, S. aurantiaca x G. superba 'Lutea', L. modesta x G. 'Marron Gold', S. aurantiaca x G. 'Marron Gold' and G. 'Verschild' x S. aurantiaca. Total DNA of one parent was labeled with digoxigenin or biotin and used as probe, and chromosomes were counterstained with 4'-6-diamidono-2-phenylindole (DAPI). For all the nine intergeneric hybrids, chromosomes from each parent could be clearly distinguished by GISH analysis. Thus GISH analysis is a powerful tool for identifying the genome constitution of intergeneric hybrids in colchicaceous ornamentals. The results obtained by GISH analysis study may be important for further progress in breeding of colchicaceous ornamentals.