Magnetic resonance spectroscopy of normal and diseased muscles.
Magnetic resonance spectroscopy of normal and diseased muscles.
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正常和患病肌肉的磁共振波谱。
DOI：
10.1002/ajmg.1320250408
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Buist,NM
中科院分区：
文献类型：
--
作者：
Chance,B;Younkin,DP;Kelley,R;Bank,WJ;Berkowitz,HD;Argov,Z;Donlon,E;Boden,B;McCully,K;Buist,NM
磷磁共振波谱(P-MRS)为研究正常和病变肌肉的氧化酶含量提供了一种创新的方法。提供了用运动原蛋白评价正常肌肉酶含量的例子。该方案提供了一个双曲线的工作/成本分布，Vmax是通过给出酶含量和“有效米氏常数”的倒数曲线图来计算的，并对静止代谢进行了评估。这一稳态方案一方面清楚地说明了酶的适应性，另一方面也清楚地说明了组织萎缩，特别是在组织损伤、杜兴氏营养不良和特定酶的遗传缺失的情况下。该方法是快速、安全的，并提供了疾病过程的定量评估和后续适当治疗的可能性。在过去的四年里，到目前为止，我们实验室已经进行了大约1000例正常和患病的人体肢体的检查。
Phosphorus magnetic resonance spectroscopy (P MRS) affords and innovative approach to the study of the oxidative enzyme content of normal and diseased muscles. Examples of the evaluation of the enzyme content of normal muscles by an exercise protoocol are provided. The protocol affords a hyperbolic work/cost profile, the Vmaxof which is calculated by the reciprocal plots giving the enzyme content and the “effective Michaelis constant” with an evaluation of the resting metabolism. This steady state protocol clearly illustrates enzyme adaptation, on the one hand, and tissue atrophy particularly in the case of tissue injury, Duchenne's dystrophy, and genetic deletion of specific enzymes, on the other hand. The method is rapid, safe, and affords a quantitative evaluation of the disease process and possibilities for following appropriate therapies. So far, approx 1000 examinations or normal and diseased human limbs have been carried out in our laboratory in over the past four years.