Electrophysiological assessment of retinal ganglion cell function.
Electrophysiological assessment of retinal ganglion cell function.
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DOI：
10.1016/j.exer.2015.05.008
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发表时间：
2015-12
影响因子：
3.4
通讯作者：
Porciatti V
中科院分区：
文献类型：
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作者：
Porciatti V
视网膜神经节细胞（RGC）的功能可以通过强调视网膜内层神经元活性的ERG技术的变体在青光眼的实验和遗传模型中进行非侵入性评估。最好理解的技术是响应于对比度反转光栅或棋盘格的模式视网膜电图（PERG），其选择性地取决于功能性RGC的存在。在青光眼模型中，PERG可以在RGC的组织学损失之前改变; PERG改变可以在中度IOP降低时逆转或在中度IOP升高时加剧。在特定的亮度刺激条件下，Flash-ERG显示可以反映起源于近端视网膜的电活动并且在一些实验性青光眼模型中被改变的分量（正暗视阈值响应，pSTR;负暗视阈值响应，nSTR;明视负响应，PhNR;振荡电位，OPs;多焦ERG，mfERG）。目前尚不清楚这些成分中哪一种对脑水肿损伤最敏感。RGC功能的电生理学评估似乎是实验性青光眼模型中的必要结果测量，其补充结构评估，甚至可以预测it. Neuroprotective策略可以基于导致改善RGC存活的基线电生理学功能的增强来测试。在青光眼模型中使用电生理学可以通过专门设计的仪器来促进，这些仪器允许对电生理功能进行高通量、稳健的评估。
The function of retinal ganglion cells (RGCs) can be non-invasively assessed in experimental and genetic models of glaucoma by means of variants of the ERG technique that emphasize the activity of inner retina neurons. The best understood technique is the Pattern Electroretinogram (PERG) in response to contrast-reversing gratings or checkerboards, which selectively depends on the presence of functional RGCs. In glaucoma models, the PERG can be altered before histological loss of RGCs; PERG alterations may be either reversed with moderate IOP lowering or exacerbated with moderate IOP elevation. Under particular luminance-stimulus conditions, the Flash-ERG displays components that may reflect electrical activity originating in the proximal retina and be altered in some experimental glaucoma models (positive Scotopic Threshold response, pSTR; negative Scotopic Threshold Response, nSTR; Photopic Negative Response, PhNR; Oscillatory Potentials, OPs; multifocal ERG, mfERG). It is not yet known which of these components is most sensitive to glaucomatous damage. Electrophysiological assessment of RGC function appears to be a necessary outcome measure in experimental glaucoma models, which complements structural assessment and may even predict it. Neuroprotective strategies could be tested based on enhancement of baseline electrophysiological function that results in improved RGC survival. The use of electrophysiology in glaucoma models may be facilitated by specifically designed instruments that allow high throughput, robust assessment of electrophysiological function.