Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.
Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.
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人类 mRNA 聚腺苷酸结合蛋白：核酸结合基序的进化保守性。
DOI：
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发表时间：
1987
影响因子：
14.9
通讯作者：
Raymond Pictet
中科院分区：
文献类型：
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作者：
Thierry Grange;Cezar Martins de Sa;J. Oddos;Raymond Pictet
我们已经克隆了编码人聚(A)结合蛋白的全长基因。73kd的碱基翻译产物与Poly(A)结合蛋白具有相同的MR、等电点和多肽图。DNA序列分析显示，它含有70,244个道尔顿蛋白。N末端部分与酵母聚(A)结合蛋白高度同源，具有足够的聚(A)结合活性。这个结构域由一个四倍重复的单位组成，大约有80个氨基酸存在于其他核酸结合蛋白中。与酵母蛋白一样，在C末端有一个大约150个氨基酸的序列，富含脯氨酸、丙氨酸和谷氨酰胺，它们加在一起占残基的48%。在几种脊椎动物细胞类型和包括卵子发生在内的各个发育阶段的果蝇中，都检测到与该基因相对应的2.9kb的mRNA。
We have isolated a full length cDNA (cDNA) coding for the human poly(A) binding protein. The cDNA derived 73 kd basic translation product has the same Mr, isoelectric point and peptidic map as the poly(A) binding protein. DNA sequence analysis reveals a 70,244 dalton protein. The N terminal part, highly homologous to the yeast poly(A) binding protein, is sufficient for poly(A) binding activity. This domain consists of a four-fold repeated unit of approximately 80 amino acids present in other nucleic acid binding proteins. In the C terminal part there is, as in the yeast protein, a sequence of approximately 150 amino acids, rich in proline, alanine and glutamine which together account for 48% of the residues. A 2,9 kb mRNA corresponding to this cDNA has been detected in several vertebrate cell types and in Drosophila melanogaster at every developmental stage including oogenesis.