Measuring screw-sense preference in a helical oligomer by comparison of 13C NMR signal separation at slow and fast exchange.
Measuring screw-sense preference in a helical oligomer by comparison of 13C NMR signal separation at slow and fast exchange.
复制标题
通过比较慢速和快速交换时的 13C NMR 信号分离来测量螺旋低聚物中的螺旋感应偏好。
DOI：
10.1021/ja1097034
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发表时间：
2011
影响因子：
15
通讯作者：
Solà J
中科院分区：
文献类型：
--
作者：
Solà J
虽然可以通过圆二色性检测到螺旋低聚物的快速相互转化的左手和右手构象异构体的不平等人口，但以前尚未实现构象异构体比率的精确定量。我们证明，使用一组标记的肽类似物，在缓慢和快速交换their13C NMR谱中的峰分离的简单分析允许获得螺旋构象异构体的比率的准确值。该方法报告的标签的网站上的构象的比例，因此可以用来调查螺旋构象控制的局部变化。
While an unequal population of rapidly interconverting left- and right-handed conformers of a helical oligomer can be detected by circular dichroism, precise quantification of a conformer ratio has not previously been achieved. We demonstrate, using a set of labeled peptide analogues, that simple analysis of peak separation in their13C NMR spectra at slow and fast exchange allows an accurate value for the ratio of helical conformers to be obtained. The method reports the ratio of conformers at the site of the label and can therefore be used to investigate local variations in helical conformational control.