Classical conditioning analog enhanced acetylcholine responses but reduced excitability of an identified neuron.
Classical conditioning analog enhanced acetylcholine responses but reduced excitability of an identified neuron.
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DOI：
10.1523/jneurosci.1256-11.2011
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发表时间：
2011-10-12
期刊：
影响因子：
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通讯作者：
Byrne JH
中科院分区：
文献类型：
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作者：
Lorenzetti FD;Baxter DA;Byrne JH
尽管经典条件反射和操作性条件反射在操作上是不同的，但尚不清楚这两种形式的学习在机制上是不同的还是相似的。小灰羊的摄食行为为解决这个问题提供了一个有用的模型系统。经典的和操作性的食欲行为训练都增强了进食和神经元的相关性。行为训练通过体外类似物使用分离的神经节来复制。此外，我们利用B51神经元开发了操作性条件反射的单细胞模拟，B51神经元是条件反射行为的重要表达细胞。在这里，一个经典条件反射的单细胞模拟被开发出来。乙酰胆碱（Acetylcholine, ACh）介导CS引起的神经节B51的兴奋，并模拟CS在单细胞模拟经典条件反射中的作用。ACh与多巴胺（DA）配对可降低B51的兴奋性，增加cs引起的B51兴奋，与体内和体外经典训练的结果相似。多巴胺介导神经节中的US。最后，D1R激动剂不能支持细胞模拟中的经典条件反射，而D1R介导操作性条件反射的强化。
Although classical and operant conditioning are operationally distinct, it is unclear whether these two forms of learning are mechanistically distinct or similar. Feeding behavior of Aplysia provides a useful model system for addressing this issue. Both classical and operant appetitive behavioral training enhance feeding and neuronal correlates have been identified. Behavioral training was replicated by in vitro analogs that use isolated ganglia. Moreover, a single-cell analog of operant conditioning was developed using neuron B51, a cell important for the expression of the conditioned behavior. Here, a single-cell analog of classical conditioning was developed. Acetylcholine (ACh) mediated the CS-elicited excitation of B51 in ganglia and mimicked the CS in the single-cell analog of classical conditioning. Pairing ACh with dopamine (DA), which mediates the US in ganglia, decreased the excitability of B51 and increased the CS-elicited excitation of B51, similar to results following both in vivo and in vitro classical training. Finally, a D1R agonist failed to support classical conditioning in the cellular analog, whereas D1R mediates reinforcement in operant conditioning.