Defective adipocyte differentiation in mice lacking the C/EBPbeta and/or C/EBPdelta gene.
Defective adipocyte differentiation in mice lacking the C/EBPbeta and/or C/EBPdelta gene.
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发表时间：
1997
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通讯作者：
T. Tanaka;N. Yoshida;T. Kishimoto;S. Akira
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作者：
T. Tanaka;N. Yoshida;T. Kishimoto;S. Akira
为了研究C/EBP家族成员在体内脂肪细胞分化过程中的作用，我们通过基因靶向产生了缺乏C/EBP β和/或C/EBP δ的小鼠。大约85%的C/EBPbeta（-/-）.delta（-/-）小鼠在新生儿早期死亡。到出生后20小时，野生型小鼠的肩胛间区域的棕色脂肪组织含有许多脂滴，而C/EBP β（-/-）δ（-/-）小鼠不积累脂滴。此外，与野生型小鼠相比，存活的成年C/EBP β（-/-）δ（-/-）小鼠的皮下脂肪垫重量显著降低。然而，尽管脂肪形成受损，C/EBPbeta（-/-）.delta（-/-）小鼠的这些脂肪组织仍表现出C/EBPalpha和PPARgamma的正常表达。这些结果表明，C/EBP β和C/EBP δ在体内终末脂肪细胞分化中具有协同作用。C/EBP α和PPARgamma的诱导并不总是需要C/EBP β和C/EBP δ，但是C/EBP α和PPARgamma的共表达在不存在C/EBP β和C/EBP δ的情况下不足以完全的脂肪细胞分化。
To investigate the role of C/EBP family members during adipocyte differentiation in vivo, we have generated mice lacking the C/EBPbeta and/or C/EBPdelta by gene targeting. Approximately 85% of C/EBPbeta(-/-).delta(-/-) mice died at the early neonatal stage. By 20 h after birth, brown adipose tissue of the interscapular region in wild-type mice contained many lipid droplets, whereas C/EBPbeta(-/-).delta(-/-) mice did not accumulate droplets. In addition, the epidydimal fat pad weight of surviving adult C/EBPbeta(-/-).delta(-/-) mice was significantly reduced compared with wild-type mice. However, these adipose tissues in C/EBPbeta(-/-).delta(-/-) mice exhibit normal expression of C/EBPalpha and PPARgamma, despite impaired adipogenesis. These results demonstrated that C/EBPbeta and C/EBPdelta have a synergistic role in terminal adipocyte differentiation in vivo. The induction of C/EBPalpha and PPARgamma does not always require C/EBPbeta and C/EBPdelta, but co-expression of C/EBPalpha and PPARgamma is not sufficient for complete adipocyte differentiation in the absence of C/EBPbeta and C/EBPdelta.