Protein absorption and desorption phenomena on clean metal surfaces.
Protein absorption and desorption phenomena on clean metal surfaces.
复制标题
清洁金属表面上的蛋白质吸收和解吸现象。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
R. Smith
中科院分区：
文献类型：
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作者：
D. Williams;I. Askill;R. Smith
用125i标记的蛋白在体外研究了白蛋白和纤维蛋白原在17种不同金属表面的非竞争吸附。虽然许多金属表现出与聚合物非常相似的吸附特性，但其中一些金属的吸附量要大得多。铜、金和银在这方面是最重要的，这三种金属都是面心立方的1B族金属，已知在体外和体内与许多类型的蛋白质结合。将预蛋白化金属植入大鼠体内，研究了蛋白质的解吸作用。从不同金属表面去除的蛋白质数量有相当大的差异。一般来说，那些最容易吸附蛋白质的金属往往会很快失去蛋白质，而其他金属，如铁，只有适度的吸附量，可以长时间保留大部分吸附层。
The noncompetitive adsorption of the proteins albumin and fibrinogen onto 17 different metal surfaces has been studied in vitro using 125I-labeled proteins. Although many of the metals showed very similar adsorption characteristics to polymers, several of them adsorbed considerably greater amounts. Copper, gold, and silver were the most significant in this respect, all three being face-centered cubic, group 1B metals known to bind to many types of protein in vitro and in vivo. The desorption of the proteins was studied in an in vivo model in which preproteinized metals were implanted in rats. There was considerable variations in the amounts of the proteins removed from the various metal surfaces. Generally those metals onto which protein is most readily adsorbed tend to lose the protein quickly, whereas others, such as iron, with only moderate adsorbed amounts, may retain a large part of the adsorbed layer for a long time.