Circulating Double-Stranded DNA in Plasma of Hemodialysis Patients and its Association with Iron Stores
Circulating Double-Stranded DNA in Plasma of Hemodialysis Patients and its Association with Iron Stores
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DOI：
10.7754/clin.lab.2015.141239
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发表时间：
2015-01-01
影响因子：
0.7
通讯作者：
Moresco, Rafael Noal
中科院分区：
文献类型：
--
作者：
Cichota, Luiz Carlos;Bochi, Guilherme Vargas;Moresco, Rafael Noal
背景：慢性肾脏病（CKD）以氧化应激为特征，CKD 的大部分不良反应是由铁催化的 ROS 生成介导的。尤其是 DNA，比其他蛋白质和膜脂更容易受到 ROS 的攻击。考虑到 CICD、铁代谢和 DNA 损伤之间关系的证据，本研究的目的是评估 HD 患者血浆中的游离 DNA 及其与铁状态生物标志物和肾功能的关系。 方法：对 40 名慢性血液透析 (HD) 患者和 40 名健康对照者进行血浆循环游离 DNA、铁、铁蛋白、转铁蛋白和其他生化参数的测量。在 HD 治疗前 1 小时和治疗后 1 小时采集血液样本，以检查单次 HD 治疗是否能够促进血浆中游离 DNA 的增加。结果：与健康对照相比，HD 患者血浆中游离 DNA 显着增加（p = 0.0017），并且观察到游离 DNA 与 GFR 和铁蛋白之间存在显着相关性。我们的研究结果表明，单次 HD 疗程无法促进游离 DNA 的增加。据报道，铁蛋白水平升高和 GFR 降低与循环游离 DNA 升高相关。结论：HD 患者的游离细胞 DNA 升高。此外，铁蛋白水平的升高和GFR的降低与DNA损伤有关。我们还观察到，单次 HD 疗程无法促进游离 DNA 的增加。
Background: Chronic kidney disease (CKD) is characterized by oxidative stress, and most of the adverse effects of CKD are mediated by iron-catalyzed ROS generation. The DNA, in particular, is more susceptible to attack by ROS than other proteins and membrane lipids. Considering the evidence on the relationship between CICD, iron metabolism, and DNA damage, the purpose of this study was to evaluate cell-free DNA in the plasma of HD patients and its association with iron status biomarkers and kidney function.Methods: Measurements of the circulating cell-free DNA in plasma, iron, ferritin, transferrin and other biochemical parameters were performed in 40 chronic hemodialysis (HD) patients and 40 healthy controls. Blood samples were also collected 1 hour before and 1 hour after the HD session to check whether a single HD session would be able to promote an increase in cell-free DNA in the plasma.Results: Cell-free DNA in plasma was significantly increased in HD patients in comparison with healthy controls (p = 0.0017), and significant correlations were observed between cell-free DNA and GFR and ferritin. Our findings showed that a single HD session was not able to promote an increase in cell-free DNA. It was reported that increased ferritin levels and reduced GFR were associated with higher circulating cell-free DNA.Conclusions: The HD patients presented increased cell-free DNA. In addition, the increase of ferritin levels and the decrease of GFR were associated with DNA damage. We also observed that a single HD session was not able to promote an increase in cell-free DNA.