Cell autonomous and nonautonomous requirements for Delltalike1 during early mouse retinal neurogenesis.
Cell autonomous and nonautonomous requirements for Delltalike1 during early mouse retinal neurogenesis.
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早期小鼠视网膜神经发生过程中 Delltalike1 的细胞自主和非自主需求。
DOI：
10.1002/dvdy.24402
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Brown,NadeanL
中科院分区：
文献类型：
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作者：
Riesenberg,AmyN;Brown,NadeanL
在脊椎动物的视网膜中，六种神经元细胞和一种神经胶质细胞是由一个共同的祖细胞池产生的。在神经发生过程中，相邻的视网膜细胞使用Notch信号通过阻止特定细胞过早分化来维持祖细胞池。在小鼠体内存在多个Notch通路的配体和受体，但每个平行通路在视网膜组织发生中的作用(S)仍然只有部分确定。结果我们分析了产前视网膜发生过程中细胞对Deltalike1(Dll1)配体的自主和非自主需求。我们使用α-Cre驱动程序同时删除一个D111条件等位基因并激活Z/EG报告基因，然后量化这个α-Cre GFP标记的谱系内外的D111突变表型。我们发现，D111活性是Hes1表达所必需的，无论是自主的还是非自主的，但令人惊讶的是，视网膜神经节细胞的分化只被细胞自主地阻止。此外，D111在视锥细胞光感受器神经发生过程中不起作用。结论虽然D111参与双向(顺式+反式)Notch信号调节Hes1的表达，但它仅自主地(在顺式)作用于解释来自其他细胞的抑制信号，从而阻断RGC的神经发生.发育动力学245：631-640,2016.©2016 Wiley期刊，Inc.
BackgroundIn the vertebrate retina, six neuronal and one glial cell class are produced from a common progenitor pool. During neurogenesis, adjacent retinal cells use Notch signaling to maintain a pool of progenitors by blocking particular cells from differentiating prematurely. In mice there are multiple Notch pathway ligands and receptors, but the role(s) of each paralogue during retinal histogenesis remains only partially defined.ResultsHere we analyzed the cell autonomous and nonautonomous requirements for theDeltalike1(Dll1) ligand during prenatal retinogenesis. We used the α‐Cre driver to simultaneously delete aDll1conditional allele and activate the Z/EG reporter, then quantifiedDll1mutant phenotypes within and outside of this α‐Cre GFP‐marked lineage. We found thatDll1activity is required for Hes1 expression, both autonomously and nonautonomously, but were surprised that retinal ganglion cell differentiation is only blocked cell autonomously. Moreover,Dll1does not act during cone photoreceptor neurogenesis. Finally,Dll1mutant adult retinas contained small retinal rosettes and RGC patterning defects but were otherwise normal.ConclusionsAlthoughDll1participates in bidirectional (cis + trans) Notch signaling to regulate Hes1 expression, it only acts cell autonomously (in cis) to interpret inhibitory signals from other cells that block RGC neurogenesis.Developmental Dynamics 245:631–640, 2016. © 2016 Wiley Periodicals, Inc.