Danggui‐shaoyao‐san, a traditional Chinese medicine prescription, alleviates the orthodontic pain and inhibits neuronal and microglia activation
Danggui‐shaoyao‐san, a traditional Chinese medicine prescription, alleviates the orthodontic pain and inhibits neuronal and microglia activation
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DOI：
10.3760/cma.j.issn.0366-6999.20141589
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发表时间：
2014-10
影响因子：
6.1
通讯作者：
Hongshi Li;Z. Gu;Li'an Wu;Liang Xia;Kecheng Zhou;L. E;Dongsheng Wang;J. Kou;Hongchen Liu
中科院分区：
文献类型：
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作者：
Hongshi Li;Z. Gu;Li'an Wu;Liang Xia;Kecheng Zhou;L. E;Dongsheng Wang;J. Kou;Hongchen Liu
研究背景正畸治疗引起的疼痛一直是困扰正畸治疗的坚韧。当归芍药散（DSS）是一种传统中药（TCM）处方，长期用于疼痛治疗，具有抗氧化，增强认知和抗抑郁作用。本研究提出DSS可能通过抑制神经元和小胶质细胞的激活而发挥其镇痛作用。方法实验性牙移动（ETM）前第5天开始每天2次给予DSS。评价定向面部梳理和空咀嚼运动（VCM）。使用免疫荧光组织化学和Western印迹分析来定量三叉神经脊束核尾侧（Vc）中的Iba-1（小胶质细胞活化）和Fos（神经元活化）表达水平。结果ETM显著增加了定向面部梳理和VCM，在术后第1天（POD）达到峰值，并在POD 7逐渐降低至基线。然而，在4小时时观察到Vc中Fos表达的急剧峰值增加，并在POD 7时逐渐降低至基线;而增加的Iba-1水平在POD 1时达到峰值，并在POD 7时逐渐降低至基线。此外，DSS预处理显著减弱了ETM诱导的定向面部梳理和VCM以及POD 1时的Fos和Iba-1水平。结论DSS对ETM诱导的疼痛具有显著的镇痛作用，并伴有神经元和小胶质细胞活化的抑制。
Background The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui‐shaoyao‐san (DSS) is a traditional Chinese medicine (TCM) prescription which has long been used for pain treatment and possesses antioxidative, cognitive enhancing and antidepressant effects. We raise the hypothesis that DSS exerts analgesic effect for orthodontic pain via inhibiting the activations of neuron and microglia. Methods DSS was given twice a day from day 5 prior to experimental tooth movement (ETM). Directed face grooming and vacuous chewing movements (VCM) were evaluated. Immunofluorescent histochemistry and Western blot analysis were used to quantify the Iba‐1 (microglia activation) and Fos (neuronal activation) expression levels in the trigeminal spinal nucleus caudalis (Vc). Results ETM significantly increased directed face grooming and VCM which reached the peak at post‐operative day (POD) 1 and gradually decreased to the baseline at POD 7. However, a drastic peak increase of Fos expression in Vc was observed at 4 hours and gradually decreased to baseline at POD 7; while the increased Iba‐1 level reached the peak at POD 1 and gradually decreased to baseline at POD 7. Furthermore, pre‐treatment with DSS significantly attenuated the ETM induced directed face grooming and VCM as well as the Fos and Iba‐1 levels at POD 1. Conclusion Treatment with DSS had significant analgesic effects on ETM‐induced pain, which was accompanied with inhibition of both neuronal and microglial activation.