Enhanced cortical responsiveness during natural sleep in freely behaving mice.
Enhanced cortical responsiveness during natural sleep in freely behaving mice.
复制标题
自由活动的小鼠在自然睡眠期间的皮质反应性增强。
DOI：
10.1038/s41598-020-59151-8
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Vogt KE.
中科院分区：
文献类型：
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作者：
Matsumoto S;Ohyama K; Diaz J;Yanagisawa M;Greene RW;Vogt KE.
皮层网络表现出大的变化，自发动力学取决于警觉状态。觉醒和快速眼动（REM）睡眠的特征在于皮层神经元的持续不规则活动，而在慢波睡眠（SWS）期间，这些神经元在沉默（OFF）和活跃（ON）期间显示同步改变。这些现象背后的网络动态尚未完全了解。关于皮层网络状态的额外信息可以通过评估睡眠-觉醒周期期间诱发的皮层反应来获得。我们测量了局部场电位（LFP）和多单位活动（MUA）在皮层中的反应，反复短暂的光遗传刺激丘脑皮层传入。LFP和MUA反应在睡眠中比清醒时显著增加，SWS期间的反应比REM睡眠期间更大。强烈增加的皮层反应在SWS相关的神经调节音，可能会减少前馈抑制的背景下进行了讨论。对刺激的反应在SWS-OFF期间比在SWS-ON期间更大。SWS反应表现出明显的昼夜波动与光暗周期相关，但对睡眠剥夺后增加的睡眠需求没有反应。潜在的稳态突触可塑性要么不存在，要么被大型警戒状态效应所掩盖。
Cortical networks exhibit large shifts in spontaneous dynamics depending on the vigilance state. Waking and rapid eye movement (REM) sleep are characterized by ongoing irregular activity of cortical neurons while during slow wave sleep (SWS) these neurons show synchronous alterations between silent (OFF) and active (ON) periods. The network dynamics underlying these phenomena are not fully understood. Additional information about the state of cortical networks can be obtained by evaluating evoked cortical responses during the sleep-wake cycle. We measured local field potentials (LFP) and multi-unit activity (MUA) in the cortex in response to repeated brief optogenetic stimulation of thalamocortical afferents. Both LFP and MUA responses were considerably increased in sleep compared to waking, with larger responses during SWS than during REM sleep. The strongly increased cortical response in SWS is discussed within the context of SWS-associated neuro-modulatory tone that may reduce feedforward inhibition. Responses to stimuli were larger during SWS-OFF periods than during SWS-ON periods. SWS responses showed clear daily fluctuation correlated to light-dark cycle, but no reaction to increased sleep need following sleep deprivation. Potential homeostatic synaptic plasticity was either absent or masked by large vigilance-state effects.