Engineered Coiled-Coil Protein Microfibers
Engineered Coiled-Coil Protein Microfibers
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DOI：
10.1021/bm5004948
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发表时间：
2014-10-01
影响因子：
6.2
通讯作者：
Montclare, Jin Kim
中科院分区：
文献类型：
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作者：
Hume, Jasmin;Sun, Jennifer;Montclare, Jin Kim
在纳米技术和医学中，制造能够在纳米到介观长度尺度上自组装的从头蛋白质在基于蛋白质的生物材料的发展中是至关重要的。在这里，我们报告的蛋白质工程卷曲螺旋的设计和表征，不仅组装成微纤维，但也可以结合疏水性小分子。在环境条件下，蛋白质形成具有纳米级结构的纤维，该纳米级结构具有由α-螺旋同五聚体组装体束形成的大纵横比，该α-螺旋同五聚体组装体束在姜黄素存在下通过聚集进一步组装成介观纤维。令人惊讶的是，这些生物合成纤维能够在非常低的浓度条件下形成。与以前设计的卷曲螺旋纤维不同，这些工程蛋白质微纤维可以在整个组装过程中结合小分子姜黄素，作为在基于蛋白质的3D微环境中封装和递送其他化学试剂的仓库
The fabrication of de novo proteins able to self-assemble on the nano- to meso-length scales is critical in the development of protein-based biomaterials in nanotechnology and medicine. Here we report the design and characterization of a protein engineered coiled-coil that not only assembles into microfibers, but also can bind hydrophobic small molecules. Under ambient conditions, the protein forms fibers with nanoscale structure possessing large aspect ratios formed by bundles of a-helical homopentameric assemblies, which further assemble into mesoscale fibers in the presence of curcumin through aggregation. Surprisingly, these biosynthesized fibers are able to form in conditions of remarkably low concentrations. Unlike previously designed coiled-coil fibers, these engineered protein microfibers can bind the small molecule curcumin throughout the assembly, serving as a depot for encapsulation and delivery of other chemical agents within protein-based 3D microenvironments