Resveratrol increases BRCA1 and BRCA2 mRNA expression in breast tumour cell lines
Resveratrol increases BRCA1 and BRCA2 mRNA expression in breast tumour cell lines
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DOI：
10.1038/sj.bjc.6600983
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发表时间：
2003-07-07
影响因子：
8.8
通讯作者：
Bernard-Gallon, DJ
中科院分区：
文献类型：
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作者：
Fustier, P;Le Corre, L;Bernard-Gallon, DJ
在葡萄、浆果和花生中发现的植物化学物质白藜芦醇，通过抑制与肿瘤发生、促进和发展相关的多种细胞事件，已被发现具有癌症化学预防作用。白藜芦醇也是一种植物雌激素，结合并激活雌激素受体，雌激素受体调节雌激素应答靶基因（如乳腺癌易感基因BRCA1和BRCA2）的转录。我们利用实时定量RT-PCR和灌注层析技术研究了白藜芦醇对人乳腺癌细胞系（MCF7、HBL 100和MDA-MB 231）中BRCA1和BRCA2表达的影响。所有细胞系均用30mum白藜芦醇处理。BRCA1和BRCA2 mrna的表达增加，但未发现蛋白表达变化。这些数据表明，30mum的白藜芦醇可以增加人乳腺肿瘤细胞系侵袭性相关基因的表达。
The phytochemical resveratrol, found in grapes, berries and peanuts, has been found to possess cancer chemopreventive effects by inhibiting diverse cellular events associated with tumour initiation, promotion and progression. Resveratrol is also a phyto-oestrogen, binds to and activates oestrogen receptors that regulate the transcription of oestrogen-responsive target genes such as the breast cancer susceptibility genes BRCA1 and BRCA2. We investigated the effects of resveratrol on BRCA1 and BRCA2 expression in human breast cancer cell lines (MCF7, HBL 100 and MDA-MB 231) using quantitative real-time RT-PCR, and by perfusion chromatography of the proteins. All cell lines were treated with 30 muM resveratrol. The expressions of BRCA1 and BRCA2 mRNAs were increased although no change in the expression of the proteins were found. These data indicate that resveratrol at 30 muM can increase expression of genes involved in the aggressiveness of human breast tumour cell lines.