Mutations in cardinal are responsible for the red-1 and peach eye color mutants of the red flour beetle Tribolium castaneum
Mutations in cardinal are responsible for the red-1 and peach eye color mutants of the red flour beetle Tribolium castaneum
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DOI：
10.1016/j.bbrc.2020.05.214
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发表时间：
2020-08-20
影响因子：
3.1
通讯作者：
Daimon, Takaaki
中科院分区：
文献类型：
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作者：
Shirai, Yu;Daimon, Takaaki
奥莫色素是昆虫眼睛、卵、翅膀和表皮中发现的主要色素。在这里，我们报告了负责 Tribolium 的 red-1 位点的基因的鉴定和表征，该基因的突变体由于眼睛中缺乏单色色素而具有白色的眼睛。使用候选基因方法，我们证明了 red-1 和 peach 突变体的主要基因存在分子缺陷，该基因编码血红素过氧化物酶，该酶被认为可将 3-羟基犬尿氨酸转化为色素颗粒中的奥莫色素。我们的实验表明，基数的表达模式与蛹阶段眼睛色素沉着的进展密切相关。我们使用受体介导的卵巢货物转导（ReMOT）控制技术进行基因编辑实验，通过成体注射破坏主要基因，并能够建立一种新的主要突变系。我们的互补测试提供了明确的遗传证据，表明基数位于 red-1 基因座。本研究将有助于更好地了解昆虫中色素途径基因的功能和多样性。我们在甲虫中成功使用 ReMOT Control 将有助于通过简单的成虫注射来开发更高效、更通用的昆虫基因组编辑系统。 (C) 2020 Elsevier Inc. 保留所有权利。
Ommochromes are the major pigments found in the eyes, eggs, wings and epidermis of insects. Here, we report the identification and characterization of the gene responsible for red-1 locus of Tribolium, whose mutants have white eyes due to lack of ommochrome pigments in the eyes. Using a candidate gene approach, we demonstrated that red-1 and peach mutants have molecular defects in the cardinal gene, which encodes a haem peroxidase that is considered to convert 3-hydroxykynurenine into ommochromes in pigment granules. Our experiments showed that the expression pattern of cardinal correlates well with the progression of eye pigmentation during pupal stages. We performed gene editing experiments using the Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control technique to disrupt the cardinal gene by adult injection, and were able to establish a novel cardinal mutant line. Our complementation test provided definitive genetic evidence that cardinal is located at the red-1 locus. The present study will lead to a greater understanding of the function and diversity of ommochrome pathway genes in insects. Our successful use of ReMOT Control in beetles will facilitate the development of more efficient and versatile systems for insect genome editing by simple adult injection. (C) 2020 Elsevier Inc. All rights reserved.