SEQUENTIAL MORPHOLOGICAL-CHANGES IN PANCREATIC-ISLETS OF SPONTANEOUSLY DIABETIC RATS
SEQUENTIAL MORPHOLOGICAL-CHANGES IN PANCREATIC-ISLETS OF SPONTANEOUSLY DIABETIC RATS
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DOI：
10.1097/00006676-199009000-00007
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发表时间：
1990-09-01
期刊：
影响因子：
2.9
通讯作者：
GAGLIARDINO, JJ
中科院分区：
文献类型：
--
作者：
DUMM, CLAG;SEMINO, MC;GAGLIARDINO, JJ
本研究描述了胰腺胰岛的连续形态学变化，从1，6，和18个月大的雄性eSS大鼠相比，年龄匹配的对照动物。口服葡萄糖耐量试验后，在6个月和18个月大的eSS大鼠中证实了自发性糖尿病。光镜免疫细胞化学和形态计量技术被用来研究胰岛细胞群。胰腺是正常的，形态测量方法没有显示1个月大的大鼠胰岛的显着变化。然而，6个月大的eSS动物显示胰岛结构破坏和间质纤维化。内分泌组织的体积密度（Vvi）、B细胞的体积密度和百分比增加，而外分泌组织的体积密度、A细胞的体积密度和百分比减少。18月龄的eSS大鼠也表现出明显的胰岛病变。内分泌组织的Vvi、B细胞的Vvi和百分比降低，外分泌组织的Vvi、D细胞的Vvi和百分比升高。我们的研究结果为糖尿病动物模型胰岛中发生的连续形态学事件提供了进一步的定量证据。
This study describes the sequential morphological changes in pancreaticislets from 1-, 6-, and 18-month-old male eSS rats, as compared to aged-matched control animals. Spontaneous diabetes mellitus was confirmed in 6-and 18-month-old eSS rats after an oral glucose tolerance test. Lightmicroscopic immunocytochemical and morphometric techniques were used to study islet-cell populations. The pancreas was normal, and the morphometric methods did not reveal significant changes in islets from 1-month-old rats. However, 6-month-old eSS animals showed disruption of islet architecture and fibrosis in the stroma. The volume density (Vvi) of endocrine tissue and the Vvi and percentage of B cells were increased, whereas the Vvi of exocrinetissue and the Vvi and percentage of A cells were diminished. Eighteen month-old eSS rats also exhibited conspicuous islet lesions. Nevertheless, the Vvi of endocrine tissue and the Vvi and percentage of B cells were diminished, while the Vvi of exocrine tissue and the Vvi and percentage of D cells were increased. Our results provide further quantitative evidence for the sequentialmor phological events occurring in the pancreatic islets of a useful animalmodel of diabetes mellitus.