The developmental proteome of Drosophila melanogaster.
The developmental proteome of Drosophila melanogaster.
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DOI：
10.1101/gr.213694.116
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发表时间：
2017-07
期刊：
影响因子：
7
通讯作者：
Butter F
中科院分区：
文献类型：
--
作者：
Casas-Vila N;Bluhm A;Sayols S;Dinges N;Dejung M;Altenhein T;Kappei D;Altenhein B;Roignant JY;Butter F
黑腹果蝇是发育生物学中广泛使用的遗传模式生物。虽然这种模式生物已经在RNA水平上进行了深入研究，但仍然缺少涵盖整个生命周期的全面蛋白质组学研究。在这里，我们应用无标记的定量蛋白质组学探索果蝇的生命周期中的蛋白质组重塑，产生7952个蛋白质，并提供了一个高时间分辨的胚胎发生蛋白质组的5458个蛋白质。我们的蛋白质组数据使我们能够在发育过程中监测34个基因的异构体特异性表达，鉴定假基因Cyp9f3 β作为蛋白质编码基因，并获得268个小蛋白质的证据。此外，与现有转录组学数据的比较揭示了mRNA和蛋白质之间相关性较差的例子，强调了蛋白质组学研究发育进程的重要性。我们的胚胎发生蛋白质组与组织特异性数据的数据整合揭示了空间和时间信息，为进一步的功能研究，但未知的蛋白质。总的来说，我们的高分辨率蛋白质组提供了一个强大的资源，可以在我们的交互式Web界面中详细探索。
Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to explore proteome remodeling across Drosophila’s life cycle, resulting in 7952 proteins, and provide a high temporal-resolved embryogenesis proteome of 5458 proteins. Our proteome data enabled us to monitor isoform-specific expression of 34 genes during development, to identify the pseudogene Cyp9f3Ψ as a protein-coding gene, and to obtain evidence of 268 small proteins. Moreover, the comparison with available transcriptomic data uncovered examples of poor correlation between mRNA and protein, underscoring the importance of proteomics to study developmental progression. Data integration of our embryogenesis proteome with tissue-specific data revealed spatial and temporal information for further functional studies of yet uncharacterized proteins. Overall, our high resolution proteomes provide a powerful resource and can be explored in detail in our interactive web interface.