Sugar and Auxin Signaling Pathways Respond to High-Temperature Stress during Anther Development as Revealed by Transcript Profiling Analysis in Cotton
Sugar and Auxin Signaling Pathways Respond to High-Temperature Stress during Anther Development as Revealed by Transcript Profiling Analysis in Cotton
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DOI：
10.1104/pp.113.232314
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发表时间：
2014-03-01
期刊：
影响因子：
7.4
通讯作者：
Zhang, Xianlong
中科院分区：
文献类型：
--
作者：
Min, Ling;Li, Yaoyao;Zhang, Xianlong
开花植物的雄性生殖对高温非常敏感。为了研究棉花花药对高温响应的分子机制，对棉花品系84021和H05在常温和高温条件下的花药发育过程进行了比较完整的转录组分析。总共筛选了4599个差异表达基因；差异表达基因主要与表观遗传修饰、碳水化合物代谢和植物激素信号转导有关。详细研究表明，在H05中，S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE1的缺乏和甲基转移酶的抑制导致了全基因组的低甲基化，而在84021中，组蛋白构成基因的表达增加导致了DNA的稳定。此外，高温诱导了H05中酪蛋白KINASEI （GhCKI）的表达，同时抑制了淀粉合酶活性，降低了花药发育过程中的葡萄糖水平，增加了花药后期的吲哚-3-乙酸（IAA）水平。在拟南芥（Arabidopsis thaliana） GhCKI过表达系中也观察到相同的变化。这些结果表明GhCKI、糖和生长素可能是花药对高温胁迫反应的关键调节因子。此外，参与糖与生长素连接并受糖调控的光敏色素相互作用因子基因（PHYTOCHROME-INTERACTING FACTOR, PIFs）可能正调控棉花药对高温胁迫的IAA生物合成。此外，外源IAA的应用表明，在高温胁迫下，高背景IAA可能对后期棉花花药不利。总之，HT、糖、pif和IAA的联系，以及我们之前报道的GhCKI数据，可能提供了植物花药对HT胁迫反应的动态协调。
Male reproduction in flowering plants is highly sensitive to high temperature (HT). To investigate molecular mechanisms of the response of cotton (Gossypium hirsutum) anthers to HT, a relatively complete comparative transcriptome analysis was performed during anther development of cotton lines 84021 and H05 under normal temperature and HT conditions. In total, 4,599 differentially expressed genes were screened; the differentially expressed genes were mainly related to epigenetic modifications, carbohydrate metabolism, and plant hormone signaling. Detailed studies showed that the deficiency in S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE1 and the inhibition of methyltransferases contributed to genome-wide hypomethylation in H05, and the increased expression of histone constitution genes contributed to DNA stability in 84021. Furthermore, HT induced the expression of CASEIN KINASEI (GhCKI) in H05, coupled with the suppression of starch synthase activity, decreases in glucose level during anther development, and increases in indole-3-acetic acid (IAA) level in late-stage anthers. The same changes also were observed in Arabidopsis (Arabidopsis thaliana) GhCKI overexpression lines. These results suggest that GhCKI, sugar, and auxin may be key regulators of the anther response to HT stress. Moreover, PHYTOCHROME-INTERACTING FACTOR genes (PIFs), which are involved in linking sugar and auxin and are regulated by sugar, might positively regulate IAA biosynthesis in the cotton anther response to HT. Additionally, exogenous IAA application revealed that high background IAA may be a disadvantage for late-stage cotton anthers during HT stress. Overall, the linking of HT, sugar, PIFs, and IAA, together with our previously reported data on GhCKI, may provide dynamic coordination of plant anther responses to HT stress.