Quercetin reduces susceptibility to influenza infection following stressful exercise
Quercetin reduces susceptibility to influenza infection following stressful exercise
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DOI：
10.1152/ajpregu.90319.2008
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发表时间：
2008-08-01
影响因子：
2.8
通讯作者：
Gangemi, J. D.
中科院分区：
文献类型：
--
作者：
Davis, J. M.;Murphy, E. A.;Gangemi, J. D.
运动应激与上呼吸道感染的风险增加有关。我们已经证明，运动压力可以增加感染的易感性。槲皮素是一种存在于各种水果和蔬菜中的黄酮类化合物，据报道可以抑制广谱病毒的感染性和复制，并可能抵消与压力运动相关的感染易感性的增加。本研究探讨了槲皮素喂养对应激运动后流感病毒A/波多黎各/8/34（H1N1）易感性的影响。小鼠被随机分配到四个治疗组之一：运动安慰剂，运动槲皮素，对照安慰剂，或对照槲皮素。运动包括连续3天在跑步机上跑步至疲劳（类似于140分钟）。病毒攻毒前，通过灌胃给予槲皮素（12.5 mg/kg）7天。最后一次运动或休息后30分钟，小鼠（n = 23-30）鼻内接种标准剂量的流感病毒（0.04血凝单位）。每天监测小鼠的发病率（发病时间）、症状严重程度和死亡率（死亡时间），持续21天。运动应激与感染易感性增加[第5-7天发病率、死亡率和症状严重程度（P < 0.05）]相关;槲皮素抵消了与应激运动相关的感染易感性增加[第5-7天发病率、死亡率和症状严重程度（P < 0.05）]。这些数据表明，短期槲皮素喂养可能被证明是一种有效的策略，以减少压力运动对呼吸道感染易感性的影响。
Exercise stress is associated with increased risk for upper respiratory tract infection. We have shown that exercise stress can increase susceptibility to infection. Quercetin, a flavonoid present in a wide variety of fruits and vegetables, has been reported to inhibit infectivity and replication of a broad spectrum of viruses and may offset the increase in susceptibility to infection associated with stressful exercise. This study examined the effects of quercetin feedings on susceptibility to the influenza virus A/Puerto Rico/8/34 (H1N1) following stressful exercise. Mice were randomly assigned to one of four treatment groups: exercise-placebo, exercise-quercetin, control-placebo, or control-quercetin. Exercise consisted of a run to fatigue (similar to 140 min) on a treadmill for 3 consecutive days. Quercetin (12.5 mg/kg) was administered via gavage for 7 days before viral challenge. At 30 min after the last bout of exercise or rest, mice (n = 23-30) were intranasally inoculated with a standardized dose of influenza virus (0.04 hemagglutinating units). Mice were monitored daily for morbidity (time to sickness), symptom severity, and mortality (time to death) for 21 days. Exercise stress was associated with an increased susceptibility to infection [morbidity, mortality, and symptom severity on days 5-7 (P < 0.05)]; quercetin offset the increase in susceptibility to infection [morbidity, mortality, and symptom severity on days 5-7 (P < 0.05)] that was associated with stressful exercise. These data suggest that short-term quercetin feedings may prove to be an effective strategy to lessen the impact of stressful exercise on susceptibility to respiratory infection.