Neurons express proteins of the classical complement pathway in Alzheimer disease
Neurons express proteins of the classical complement pathway in Alzheimer disease
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DOI：
10.1016/s0006-8993(97)00849-4
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发表时间：
1997-09-26
期刊：
影响因子：
2.9
通讯作者：
McGeer, PL
中科院分区：
文献类型：
--
作者：
Terai, K;Walker, DG;McGeer, PL
通过免疫组织化学和蛋白质印迹研究了人海马和颞叶皮层中经典途径补体蛋白 C1q、C1r、C1s、C2、C3、C4、C5、C6、C7、C8 和 C9 的出现。在阿尔茨海默病（AD）病例中，在锥体神经元和老年斑中观察到所有这些蛋白质的阳性染色。在对照病例中，除了未检测到 C1q 和 C1s 之外，观察到较弱的锥体神经元染色。在 AD 海马提取物的蛋白质印迹中，每种补体蛋白都发现了与正常血清中检测到的条带相对应的条带。在正常海马提取物中也检测到了类似的条带，但未观察到 C1s 除外。 AD 中的条带强度通常比正常提取物中的条带强度更强，但是，在后者中，病例之间以及单个病例中的条带之间存在相当大的变异性。这些数据表明，锥体神经元可能是已知与阿尔茨海默病病变相关的补体成分的来源。 (C) 1997 Elsevier Science B.V.
Occurrence of the classical pathway complement proteins C1q, C1r, C1s, C2, C3, C4, C5, C6, C7, C8 and C9 was studied in human hippocampus and temporal cortex by immunohistochemistry and Western blotting. In Alzheimer disease (AD) cases, positive staining for all of these proteins was observed in pyramidal neurons and senile plaques. In control cases, weaker pyramidal neuron staining was observed except for C1q and C1s which were not detected. On Western blots of AD hippocampal extracts, bands corresponding to those detected in normal serum were found for each of the complement proteins. Comparable bands were also detected in normal hippocampal extracts with the exception of C1s which was not observed. The intensity of the bands was generally stronger in AD than in normal extracts, but, in the latter, there was considerable variability between cases and between bands in a single case. These data suggest that pyramidal neurons may be a source of the complement components known to be associated with Alzheimer lesions. (C) 1997 Elsevier Science B.V.