Molecular typing of Vibrio parahaemolyticus isolates from the middle-east coastline of China
Molecular typing of Vibrio parahaemolyticus isolates from the middle-east coastline of China
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中国中东海岸线副溶血性弧菌的分子分型
DOI：
10.1016/j.ijfoodmicro.2011.12.001
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发表时间：
2012-02-15
影响因子：
5.4
通讯作者：
Shi, Xianming
中科院分区：
文献类型：
--
作者：
Chen, Wanyi;Xie, Yanping;Shi, Xianming
在中国暴发的副血吸虫性胃肠炎弧菌突显了对该致病菌种进行菌株鉴定和亚型鉴定的必要性。2006年至2008年，从中国中东部海岸线的临床样品、海产品和各种环境场所中分离到56株流行病学无关的副溶血性弧菌。用核糖体分型、肠道细菌重复基因间一致性序列聚合酶链式反应(ERIC-PCR)、脉冲场凝胶电泳法(PFGE)和gyrB基因序列分析等4种分子分型方法对分离株进行鉴定。从这些分子分型试验得到的聚类分析的遗传图谱清楚地表明，来自海鲜及其环境的菌株在潜在致病性上存在差异。来自海鲜的两个分离株(F13和QS2)的基因特征表明，它们具有潜在的致病性。利用Simpson‘s多样性指数对56株副溶血性弧菌的4种分型方法的判别指数进行了区分。ERIC-PCR分型的判别指数最大(D=0.942)，gyrB基因序列分析的判别指数最小(D=0.702)。ERIC-PCR法与gyrB基因序列分析相结合，可显著提高鉴别能力(D=0.966)。这些结果表明ERIC-PCR结合gyrB基因序列分析可能是一种可靠、快速的副溶血性弧菌分型方法。(C)2011爱思唯尔B.V.保留所有权利。
The occurrence of outbreaks of Vibrio parahaemolyncus gastroenteritis in China highlights the need for strain characterization and subtyping of this pathogenic species. A total of 56 epidemiologically-unrelated strains of V. parahaemolyticus were isolated from clinical samples, seafood and various environmental sites in the middle-east coastline of China from 2006 to 2008. The isolates were characterized using four molecular typing methods, including ribotyping, enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR), pulsed-field gel electrophoresis (PFGE), and sequence analysis of the gyrB gene. Genetic profiles of cluster analysis from these molecular typing tests clearly showed that there were differences in potential pathogenicity among isolates from seafood and its environments. Genetic characterization of two isolates (F13 and QS2) that originated from seafood demonstrated that they were potentially pathogenic. Discriminatory indices of four typing methods for the 56 V. parahaemolyticus isolates were differentiated by Simpson's Index of Diversity. The discriminatory index of ERIC-PCR typing was maximal (D = 0.942), while that of sequence analysis of the gyrB gene was minimal (D = 0.702). The discriminatory ability was greatly enhanced (D = 0.966) when ERIC-PCR was coupled with sequence analysis of the gyrB gene. These results suggest that ERIC-PCR combined with sequence analysis of gyrB gene may be a reliable, rapid typing strategy for V. parahaemolyticus strains. (C) 2011 Elsevier B.V. All rights reserved.