EVIDENCE FOR A RELATIONSHIP BETWEEN LONGEVITY OF MAMMALIAN-SPECIES AND LIFE SPANS OF NORMAL FIBROBLASTS INVITRO AND ERYTHROCYTES INVIVO
EVIDENCE FOR A RELATIONSHIP BETWEEN LONGEVITY OF MAMMALIAN-SPECIES AND LIFE SPANS OF NORMAL FIBROBLASTS INVITRO AND ERYTHROCYTES INVIVO
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DOI：
10.1073/pnas.78.8.5009
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
ROHME, D
中科院分区：
文献类型：
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作者：
ROHME, D
在代表8个物种的细胞培养物中研究了体外哺乳动物成纤维细胞的复制寿命。这些包括来自小鼠、大鼠、袋鼠、水貂、兔、蝙蝠、马和人的肺、皮肤、睾丸或整个胚胎的活检。重点放在确定人口倍增水平，在第三阶段（一个时期的增殖率下降）和染色体发生变化。所有研究的细胞培养物都经历了生长危机，即持续至少2周的明显生长停止期。在大多数文化中，危机代表着它们复制能力的终结，但在某些文化中，细胞增殖在危机之后又恢复了。在生长危机之前，证明了主要的二倍体染色体构成（> 75%）。在危机后恢复细胞增殖的培养物中，除大鼠（始终有90%或更多的二倍体细胞）外，所有情况下均为非二倍体结构。增长危机发生在种群倍增水平，这是物种的特征，与物种的最大寿命呈严格的幂律关系，与最大寿命的平方根成正比。根据文献数据，相同的关系适用于体内循环哺乳动物红细胞的寿命。显然，有一个共同的功能基础，调节成纤维细胞和红细胞的寿命，从而在体外和体内的复制以及有丝分裂后的细胞。
The replicative life spans of mammalian fibroblasts in vitro were studied in cell cultures representing 8 species. These included biopsy lung, skin, testis or whole embryos from mouse, rat, rat kangaroo, mink, rabbit, bat, horse and humans. Emphasis was placed on determining the population doubling level at which phase III (a period of decrease in the rate of proliferation) and chromosomal alterations occur. All the cell cultures studied went through a growth crisis, a period of apparent growth cessation lasting for at least 2 wk. In most cultures the crisis represented the end of their replicative capacities, but in some cultures cell proliferation was resumed after the crisis. A predominantly diploid chromosome constitution (> 75%) was demonstrated prior to the growth crisis. In cultures in which cell proliferation was resumed after the crisis, a nondiploid constitution prevailed in all cases except the rat (with 90% or more diploid cells all the time). The growth crisis occurred at population doubling levels that were characteristic for the species and was related to the species'' maximal life span by a strict power law, being proportional to the square root of the maximal life span. Based on literature data, the same relationship was valid for the lifespans of circulating mammalian erythrocytes in vivo. Apparently, there is a common functional basis regulating the life span of fibroblasts and erythrocytes and thus operating in replicative as well as postmitotic cells in vitro and in vivo.