Dynamics of Th17 associating cytokines in Cryptosporidium parvum-infected mice
Dynamics of Th17 associating cytokines in Cryptosporidium parvum-infected mice
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小隐孢子虫感染小鼠中 Th17 相关细胞因子的动态
DOI：
10.1007/s00436-015-4831-2
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发表时间：
2016-02-01
影响因子：
2
通讯作者：
Lin, Q.
中科院分区：
文献类型：
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作者：
Zhao, G. H.;Fang, Y. Q.;Lin, Q.
隐孢子虫通常栖息于动物和人类的肠道，可引起急性水样腹泻和体重减轻。然而，宿主对隐孢子虫感染的免疫反应尚未完全了解。IL-17（也称为IL-17 A）是Th 17细胞的促炎细胞因子，其在宿主对贝氏隐孢子虫感染的应答中起作用。本研究检测了C.使用实时定量PCR（qPCR）检测细小病毒感染的免疫抑制BALB/c小鼠。通过ELISA测定IL-17蛋白水平。结果显示，肠相关淋巴组织（GALT）和脾脏中IL-17 mRNA和Th 17细胞相关细胞因子TGF-β、IL-6、STAT-3、RORγt、IL-22、TNF-α和IL-23的水平显著升高（P 0.05）。感染后GALT中IL-17蛋白水平也显著升高（P0.05）.本研究提示Th 17细胞在宿主-C中发挥作用。微小相互作用这些结果可以为将来研究抗C.短暂免疫抑制人群中的细小病毒感染。
Cryptosporidium parvum commonly inhabits the intestinal tract of animals and humans and can cause acute watery diarrhea and weight loss. However, host immune responses to Cryptosporidium infections are not fully understood. IL-17 (also called IL-17A) is a pro-inflammatory cytokine of Th17 cells that plays a role in the host response to Cryptosporidium baileyi infection. The present study examined levels of IL-17-specific messenger RNA (mRNA) and Th17 associating cytokines in C. parvum-infected immune-suppressed BALB/c mice using real-time quantitative PCR (qPCR). Levels of IL-17 protein were determined by ELISA. The results showed that levels of IL-17 mRNA and Th17 cell-related cytokines, namely TGF-β, IL-6, STAT-3, RORγt, IL-22, TNF-α, and IL-23, were significantly increased (P < 0.05) in gut-associated lymphoid tissue (GALT) and spleen. IL-17 protein levels in GALT were also significantly increased (P < 0.05) after infection. The present study suggested that Th17 cells play a role in host-C. parvum interaction. These results could inform future studies of the immune response against C. parvum infection in transient immunosuppressed populations.