Absolute Quantification of Brain Creatine Concentration Using Long Echo Time PRESS Sequence with an External Standard and LCModel: Verification with in Vitro HPLC Method
Absolute Quantification of Brain Creatine Concentration Using Long Echo Time PRESS Sequence with an External Standard and LCModel: Verification with in Vitro HPLC Method
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DOI：
10.1007/11893028_23
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发表时间：
2006-10
期刊：
影响因子：
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通讯作者：
Y. Lin;Y. P. Zhang;Renhua Wu;Hui Li;Zhiwei Shen;X. Chen;K. Huang;G. Guo
中科院分区：
文献类型：
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作者：
Y. Lin;Y. P. Zhang;Renhua Wu;Hui Li;Zhiwei Shen;X. Chen;K. Huang;G. Guo
目的：探讨在体长回波时间PRESS序列外标法定量测定脑组织肌酸（Cr）浓度的准确性。10头猪和1个外标体模，用1.5T GE Signa扫描仪和标准头部线圈，用PRESS序列采集2个VOI（2 × 2 × 2cm ~ 3）和外标液的1H-MRS数据，TE = 135 ms，TR = 1500 ms，平均128次扫描。通过LCModel进行体内Cr评价。采用高效液相色谱法分析体外培养液中铬的浓度。1H-MRS组Cr浓度为9.37±0.137 mmol/kg。HPLC组Cr浓度为8.905±0.126 mmol/kg。两种方法检测结果的一致性较好（P=0.491），表明长回波时间PRESS序列加外标能准确检测脑Cr浓度。LCModel的应用为MRS的量化提供了方便。
To investigate the accuracy for absolute quantification of brain creatine (Cr) concentration using in vivo long echo time PRESS sequence performed with an external standard. Ten swine and an external standard phantom were investigated by 1.5T GE Signa scanner and the standard head coil.1H-MRS data were acquired from the two VOI (2x2x2cm3) placed in swine brain and external standard solution by using PRESS sequence with TE = 135 mses, TR = 1500 msec, and 128 scan averages. In vivo Cr evaluation was made by LCModel. In vitro Cr concentration was analyzed by HPLC method. In the1H-MRS group, the Cr concentration was 9.37±0.137 mmol/kg. In the HPLC group, the Cr concentration was 8.905±0.126 mmol/kg. Good agreement was obtained between in these two methods (P=0.491), which indicated that long echo time PRESS sequence with an external standard can accurately detect brain Cr concentration. The application of LCModel introduces more convenience for the MRS quantification.