Inertial cavitation dose and hemolysis produced in vitro with or without Optison®
Inertial cavitation dose and hemolysis produced in vitro with or without Optison®
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DOI：
10.1016/s0301-5629(03)00013-9
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发表时间：
2003-05-01
影响因子：
2.9
通讯作者：
Crum, LA
中科院分区：
文献类型：
--
作者：
Chen, WS;Brayman, AA;Crum, LA
气基造影剂(CA)增加超声(US)诱导的生物效应，可能是通过惯性空化(IQ机制)。用Optison(R)研究了IC剂量(ICD)(累积均方根[RMS]宽带噪声幅度；频域)与1.1 MHz超声诱导的人全血溶血之间的关系；假设溶血与ICD相关。进行了四个实验系列，变量：1.峰值负声压(P-)，2.Optison(R)浓度，3.脉冲宽度和4.总暴露时间和Optison(R)浓度。溶血和ICD的P阈值类似于0.5兆帕。当Optison(R)浓度大于或等于0.01V%，且脉冲持续时间低至四个或两个循环时，分别检测到ICD和溶血。溶血率和ICD是时间和Optison(R)浓度的函数；最终溶血率和ICD值取决于初始Optison(R)浓度，但初始变化率不是。在系列内，溶血与ICD显著相关；在P<；0.001，这种相关性显著。(C)2003年世界医学生物学超声联合会。
Gas-based contrast agents (CAs) increase ultrasound (US)-induced bioeffects, presumably via an inertial cavitation (IQ mechanism. The relationship between IC dose (ICD) (cumulated root mean squared [RMS] broadband noise amplitude; frequency domain) and 1.1-MHz US-induced hemolysis in whole human blood was explored with Optison(R); the hypothesis was that hemolysis would correlate with ICD. Four experimental series were conducted, with variable: 1. peak negative acoustic pressure (P-), 2. Optison(R) concentration, 3. pulse duration and 4. total exposure duration and Optison(R) concentration. P- thresholds for hemolysis and ICD were similar to0.5 MPa. ICD and hemolysis were detected at Optison(R) concentrations greater than or equal to 0.01 V%, and with pulse durations as low as four or two cycles, respectively. Hemolysis and ICD evolved as functions of time and Optison(R) concentration; final hemolysis and ICD values depended on initial Optison(R) concentration, but initial rates of change did not. Within series, hemolysis was significantly correlated with ICD; across series, the correlation was significant at p < 0.001. (C) 2003 World Federation for Ultrasound in Medicine Biology.