Phylogenetic utility of the AP3/DEF K-domain and its molecular evolution in Impatiens (Balsaminaceae)
Phylogenetic utility of the AP3/DEF K-domain and its molecular evolution in Impatiens (Balsaminaceae)
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DOI：
10.1016/j.ympev.2006.11.016
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发表时间：
2007-04-01
影响因子：
4.1
通讯作者：
Smets, Erik
中科院分区：
文献类型：
--
作者：
Janssens, Steven;Geuten, Koen;Smets, Erik
APETALA 3（AP 3）/DEFICIENS（DET）是一个MADS盒转录因子，参与花瓣和雄蕊花器官的鉴定。AP 3/DEF基因谱系已在被子植物中广泛研究，以更好地了解其在花多样性和进化中的作用。因此，大量克隆的AP 3/DEF直向同源物是可用的，其可用于设计分类特异性引物，用于感兴趣的组的近亲的生殖重建。根据这一推理，我们研究了最近在凤仙花属（凤仙花科）中发现的两个AP 3/DEF旁系同源物（ImpDEF 1和ImpDEF 2）的系统发育效用。对59种凤仙花属植物的AP 3/DEF重复序列的K-结构域内含子4和5进行扩增和测序。系统发育分析的分离和合并的ImpDEF 1和ImpDEF 2数据集的结果高度一致的拓扑结构与先前获得的叶绿体atpB-rbcL数据集。叶绿体和核基质的组合结果是一个很好的支持进化假说的凤仙花。我们的研究结果表明，内含子4和5的AP 3/DEF样基因是一个有价值的来源的字符的系统发育研究在属下水平。(c)2006年爱思唯尔公司All rights reserved.
APETALA3 (AP3)/DEFICIENS (DET) is a MADS-box transcription factor that is involved in establishing the identity of petal and stamen floral organs. The AP3/DEF gene lineage has been extensively examined throughout the angiosperms in order to better understand its role in floral diversity and evolution. As a result, a large number of cloned AP3/DEF orthologues are available, which can be used for the design of taxon specific primers for phylogeny reconstruction of close relatives of the group of interest. Following this reasoning, we investigated the phylogenetic utility of the two AP3/DEF paralogues (ImpDEF1 and ImpDEF2) that were recently identified in the genus Impatiens (Balsaminaceae). K-domain introns 4 and 5 of both AP3/DEF duplicates were amplified and sequenced for 59 Impatiens species. Phylogenetic analyses of the separated and combined ImpDEF1 and ImpDEF2 data sets result in highly congruent topologies with the previously obtained chloroplast atpB-rbcL data set. Combination of chloroplast and nuclear matrices results in a well-supported evolutionary hypothesis of Impatiens. Our results show that introns 4 and 5 in AP3/DEF-like genes are a valuable source of characters for phylogenetic studies at the infrageneric level. (c) 2006 Elsevier Inc. All rights reserved.