Presence of genes for type III secretion system 2 in Vibrio mimicus strains.
Presence of genes for type III secretion system 2 in Vibrio mimicus strains.
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DOI：
10.1186/1471-2180-10-302
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发表时间：
2010-11-29
期刊：
影响因子：
4.2
通讯作者：
Iida T
中科院分区：
文献类型：
--
作者：
Okada N;Matsuda S;Matsuyama J;Park KS;de los Reyes C;Kogure K;Honda T;Iida T
弧菌包括100多个种类，在海洋和河口环境中普遍存在，其中几种例如霍乱弧菌、副溶血弧菌、创伤弧菌和拟态弧菌是人类的病原体。致病性副溶血性弧菌菌株具有用于III型分泌系统（T3 SS）的两组基因，T3 SS 1和T3 SS 2。后者对微生物的毒力至关重要，并被分为两个不同的群，T3 SS 2 α和T3 SS 2 β，据报道也在致病性霍乱弧菌非O 1/非O 139血清群菌株中发现。然而，T3 SS 2相关基因是否存在于其他弧菌物种中仍不清楚。因此，我们通过使用靶向T3 SS 2 α和T3 SS 2 β基因的PCR检测来检查T3 SS 2基因在副溶血性弧菌以外的弧菌中的分布。在我们研究测试的32种弧菌中，在霍乱弧菌、拟态弧菌和霍氏弧菌这三种弧菌中检测到了多个T3 SS 2相关基因，并且大多数III型分泌必需基因都存在于T3 SS 2阳性的霍乱弧菌和拟态弧菌菌株中。此外，还鉴定了具有T3 SS 2 α和T3 SS 2 β的拟态弧菌菌株。拟态弧菌菌株中T3 SS 2基因簇的基因组织与副溶血性弧菌和霍乱弧菌中具有T3 SS 2 α和T3 SS 2 β的菌株基本相似。这项研究是首次报道的拟态弧菌菌株中存在T3 SS 2基因簇的证据。因此，这一发现为研究拟态弧菌的致病性提供了新的视角。
Vibrios, which include more than 100 species, are ubiquitous in marine and estuarine environments, and several of them e.g. Vibrio cholerae, V. parahaemolyticus, V. vulnificus and V. mimicus, are pathogens for humans. Pathogenic V. parahaemolyticus strains possess two sets of genes for type III secretion system (T3SS), T3SS1 and T3SS2. The latter are critical for virulence of the organism and be classified into two distinct phylogroups, T3SS2α and T3SS2β, which are reportedly also found in pathogenic V. cholerae non-O1/non-O139 serogroup strains. However, whether T3SS2-related genes are present in other Vibrio species remains unclear. We therefore examined the distribution of the genes for T3SS2 in vibrios other than V. parahaemolyticus by using a PCR assay targeting both T3SS2α and T3SS2β genes. Among the 32 Vibrio species tested in our study, several T3SS2-related genes were detected in three species, V. cholerae, V. mimicus and V. hollisae, and most of the essential genes for type III secretion were present in T3SS2-positive V. cholerae and V. mimicus strains. Moreover, both V. mimicus strains possessing T3SS2α and T3SS2β were identified. The gene organization of the T3SS2 gene clusters in V. mimicus strains was fundamentally similar to that of V. parahaemolyticus and V. cholerae in both T3SS2α- and T3SS2β-possessing strains. This study is the first reported evidence of the presence of T3SS2 gene clusters in V. mimicus strains. This finding thus provides a new insight into the pathogenicity of the V. mimicus species.
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影响因子：
3.1
作者：
Park, KS;Ono, T;Honda, T
通讯作者：
Honda, T
影响因子：
3.6
作者：
NISHIBUCHI, M;KAPER, JB
通讯作者：
KAPER, JB
影响因子：
3.4
作者：
Kodama, Toshio;Rokuda, Mitsuhiro;Honda, Takeshi
通讯作者：
Honda, Takeshi
影响因子：
168.9
作者：
Makino, K;Oshima, K;Iida, T
通讯作者：
Iida, T
影响因子：
3.5
作者：
IIDA, T;YAMAMOTO, K
通讯作者：
YAMAMOTO, K