Identification and characterization of a protein containing formin homology (FH1/FH2) domains.
Identification and characterization of a protein containing formin homology (FH1/FH2) domains.
复制标题
含有福尔明同源 (FH1/FH2) 结构域的蛋白质的鉴定和表征。
DOI：
10.1016/s0378-1119(99)00127-4
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发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
Hiebert,SW
中科院分区：
文献类型：
--
作者：
Westendorf,JJ;Mernaugh,R;Hiebert,SW
克隆并鉴定了福尔明/透明蛋白家族的一个新成员。4kB的mRNA普遍表达，但在脾中含量丰富。FHOS(在脾中过表达的福明同源物)含有3414bp的开放阅读框，编码约128 kDa的蛋白质。FHOS与透明蛋白和福尔明蛋白在福尔明同源(FH)1和FH2结构域具有序列同源性。FHOS还含有一个卷曲的卷曲结构域、一个胶原样结构域、两个核定位信号以及几个潜在的PKC和PKA磷酸化位点。FHOS特异性抗血清被用来确定FHOS是一种主要的细胞质蛋白，在各种人类细胞系中都有表达。FHOS定位在染色体16q22上，位于骨架标记WI-5594和WI-9392之间。
A novel member of the Formin/Diaphanous family of proteins was cloned and characterized. A 4kB mRNA is ubiquitously expressed but is found in abundance in the spleen. FHOS (Formin Homologue Overexpressed in Spleen) contains a 3414bp open reading frame and encodes for an approximately 128kDa protein. FHOS has sequence homology to Diaphanous and Formin proteins within the Formin Homology (FH)1 and FH2 domains. FHOS also contains a coiled-coil, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. FHOS-specific antiserum was generated and used to determine that FHOS is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. FHOS was mapped to chromosome 16q22 between framework markers WI-5594 and WI-9392.