VESICULAR TRANSPORT OF PEROXIDASE IN HUMAN EOSINOPHILIC MYELOCYTES
VESICULAR TRANSPORT OF PEROXIDASE IN HUMAN EOSINOPHILIC MYELOCYTES
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DOI：
10.1111/j.1365-2222.1994.tb00910.x
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发表时间：
1994-01-01
影响因子：
6.1
通讯作者：
ISHIZAKA, T
中科院分区：
文献类型：
--
作者：
DVORAK, AM;ESTRELLA, P;ISHIZAKA, T
我们进行了超微结构细胞化学研究，以检测发育停滞的人嗜酸性中幼粒细胞中的过氧化物酶。在鼠源性条件培养基存在的情况下，将人脐带血单核细胞培养21天，结果产生了嗜酸性中幼粒细胞。与正常发育的嗜酸性中幼粒细胞不同，正常发育的嗜酸性中幼粒细胞在合成细胞器（即围绕细胞核且由粗面内质网和高尔基体结构界定的池）以及未成熟和成熟颗粒中均含有过氧化物酶，而发育停滞的细胞显示出过氧化物酶合成及分泌运输减少的超微结构证据。因此，在核周和粗面内质网池、高尔基体结构、未成熟颗粒以及大多数成熟颗粒的基质区室中通常不存在过氧化物酶。相反，双区室特异性颗粒呈现为空的、过氧化物酶阴性的基质以及中心的、过氧化物酶阴性的核心物质。过氧化物酶存在于颗粒周围的囊泡中，其中一些囊泡附着于颗粒上。这种负载过氧化物酶的运输囊泡类似于在含rhIL - 5培养基中培养的成熟人嗜酸性粒细胞进行的逐步脱颗粒过程中所涉及的囊泡[1]。这些发现确立了在未成熟人嗜酸性粒细胞的分泌功能中存在囊泡介导的逐步脱颗粒现象，并提示嗜酸性中幼粒细胞在体内可能参与需要从特异性颗粒基质区室进行选择性分泌的重要生理和/或病理事件的可能性。
We performed ultrastructural cytochemistry to detect peroxidase in developmentally arrested human eosinophilic myelocytes. Human umbilical cord blood mononuclear cells were cultured for 21 days in the presence of murine-derived conditioned media, resulting in the development of eosinophilic myelocytes. Unlike normally developing eosinophilic myelocytes. which contain peroxidase in synthetic organelles (i.e. cisterns surrounding the nucleus and bounded by the rough endoplasmic reticulum and Golgi structures) and in immature and mature granules, the developmentally arrested cells showed ultrastructural evidence of decreased synthesis and secretory transport of peroxidase. Thus, peroxidase was generally absent in the perinuclear and rough endoplasmic cisterns, in Golgi structures, in immature granules and in the matrix compartment of most mature granules. Rather, bicompartmental specific granules displayed empty, peroxidase-negative matrix and central, peroxidase-negative core material. Peroxidase was present in perigranular vesicles, some of which were attached to granules. Such peroxidase-loaded transport vesicles are similar to those that effect piecemeal degranulation of mature human eosinophils cultured in rhI1-5-containing media [1]. These findings establish vesicle-mediated piecemeal degranulation in the secretory repertoire of immature human eosinophils and suggest the possibility that eosinophilic myelocytes may participate in vivo in important physiological and/or pathological events that require selective secretion from the specific granule matrix compartment.