Antimicrobial properties and phytochemical analysis of Emblica officinalis
Antimicrobial properties and phytochemical analysis of Emblica officinalis
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余甘子的抗菌特性和植物化学分析
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
S. Sabnis
中科院分区：
文献类型：
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作者：
Prachi Javale;S. Sabnis
在本研究中，试图确定余甘子的果实和叶的粗粉，水以及甲醇提取物对三种常见的呼吸道病原体，即金黄色葡萄球菌，肺炎克雷伯氏菌，化脓性链球菌的抗菌活性。采用琼脂平板法对提取物进行了初步抑菌试验，并采用平板稀释法测定了提取物的最低抑菌浓度。金黄色葡萄球菌被发现是最敏感的生物体的三种病原体在研究中。结果表明，麻黄的甲醇提取物对上述3种病原菌的生化特性均有显著影响。对麻黄的初步植物化学分析表明，麻黄中含有单宁、皂苷、黄酮类化合物和酚类化合物，其中黄酮类化合物和皂苷类化合物对上述3种病原菌的抑制作用最强。进一步的研究表明，这两种植物化学物质之间的协同作用，其中发现它们的组合提取物与单独的植物化学物质相比，对测试病原体的抑制作用更强。基于本研究中获得的结果，余甘子确实对最常见的呼吸道病原体具有有效的抗菌活性，可用于治疗由这些微生物引起的各种疾病。余甘子，金黄色葡萄球菌，肺炎克雷伯菌
In the present study, an attempt was made to determine the antimicrobial activity of crude powder, aqueous as well methanolic extract of the fruit and leaf of Emblica officinalis against three commonly encountered respiratory pathogens viz. Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus pyogenes. The preliminary antimicrobial analysis was carried out using the Agar ditch plate method and the minimum inhibitory concentration of the extracts were then determined using Plate dilution technique. Staphylococcus aureus was found to be the most susceptible organism of the three pathogens in the study. Methanolic extract of E.officinalis was found to bring about major alterations in the biochemicals characteristics of all the three pathogens Preliminary phytochemical analysis of E.officinalis showed presence of tannins, saponins, flavanoids and phenols of which flavonoids and saponins were found to be the most inhibitory towards all the pathogens. Further studies showed synergism between these two phytochemicals, where their combined extract was found to be more inhibitory towards the test pathogens in comparison with individual phytochemicals alone. Based on the results obtained in the present study, Emblica officinalis definitely possesses potent antimicrobial activity against most commonly encountered respiratory pathogens and can be used in treating various diseases caused by these organisms. Emblica officinalis, , Staphylococcus aureus, Klebsiella pneumoniae