Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels
Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels
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DOI：
10.1002/jbm.1217
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发表时间：
2002-01-01
期刊：
影响因子：
--
通讯作者：
Anseth, KS
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文献类型：
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作者：
Bryant, SJ;Anseth, KS
在软骨组织工程中使用水凝胶支架时，两个凝胶特性特别重要：平衡含水量（q，平衡膨胀比）和压缩模量k。在这项工作中，软骨细胞被光封装在降解和非降解聚乙二醇基水凝胶中，以评估细胞外基质（ECM）形成作为这些凝胶特性的函数。在非降解凝胶中，当q从4.2变化到9.3时，体外培养2周和4周后，凝胶中的糖胺聚糖（GAG）含量无显著差异。然而，当q值为9.3时，GAGs可以均匀地在凝胶中扩散，而当q值小于或等于5.2时，则会导致GAGs在细胞周围的定位。有趣的是，在K为360 kPa的适度交联凝胶中，与K较高（960 kPa）和较低（30 kPa）的凝胶相比，4周后观察到II型胶原合成增加。通过将可降解键结合到网络中，获得了初始高K （350 kPa）和最终高q（7.9）的凝胶特性，从而增加了II型胶原的合成，并使gag分布均匀。因此，在形成功能性ECM时，凝胶膨胀、力学和降解之间存在一个关键的平衡。(C) 2001约翰威利父子公司
When using hydrogel scaffolds for cartilage tissue engineering, two gel properties are particularly important: the equilibrium water content (q, equilibrium swelling ratio) and the compressive modulus, K. In this work, chondrocytes were photoencapsulated in degrading and nondegrading poly(ethylene glycol)-based hydrogels to assess extracellular matrix (ECM) formation as a function of these gel properties. In nondegrading gels, the glycosaminoglycan (GAG) content was not significantly different in gels when q was varied from 4.2 to 9.3 after 2 and 4 weeks in vitro. However, gels with a q of 9.3 allowed GAGs to diffuse throughout the gels homogenously, but a q less than or equal to 5.2 resulted in localization of GAGs pericellularly. Interestingly, in the moderately crosslinked gels with a K of 360 kPa, an increase in type II collagen synthesis was observed compared with gels with a higher (960 kPa) and lower (30 kPa) K after 4 weeks. With the incorporation of degradable linkages into the network, gel properties with an initially high K (350 kPa) and final high q (7.9) were obtained, which allowed for increased type II collagen synthesis coupled with a homogenous distribution of GAGs. Thus, a critical balance exists between gel swelling, mechanics, and degradation in forming a functional ECM. (C) 2001 John Wiley & Sons, Inc.