OsαCA1 Affects Photosynthesis, Yield Potential, and Water Use Efficiency in Rice.
OsαCA1 Affects Photosynthesis, Yield Potential, and Water Use Efficiency in Rice.
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Osα CA 1影响水稻光合作用、产量潜力和水分利用效率
DOI：
10.3390/ijms24065560
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发表时间：
2023-03-14
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
考虑到二氧化碳（CO2）的可用性，植物生长和作物产量基本上由光合作用决定。CO2在叶片内的扩散是决定叶绿体中CO2浓度的因素之一。碳酸酐酶（CAs）是一种含锌的酶，可以将CO2和碳酸氢根离子（HCO 3 −）相互转化，从而影响CO2的扩散，因此在所有光合生物中起着重要作用。近年来，这一领域的研究取得了很大进展，极大地促进了人们对β-型CA功能的了解，但对植物中α-型CA的研究仍处于起步阶段。本研究通过对水稻剑叶中OsαCAs基因的表达分析及其编码蛋白的亚细胞定位，鉴定了水稻Osα CA 1基因。Osα CA 1编码一种α型CA，其蛋白质定位于叶绿体中，在光合组织（包括旗叶、成熟叶和穗）中具有高丰度。Osα CA 1缺乏导致同化率、生物量积累和籽粒产量显著降低。Osα CA 1突变体的生长和光合缺陷归因于叶绿体羧化位点的CO2供应受限，这可以通过应用高浓度的CO2而不是HCO 3 −来部分拯救。此外，我们还提供了证据表明，Osα CA 1正调控水稻水分利用效率（WUE）。综上所述，本研究结果揭示了Osα CA 1在水稻光合作用和产量潜力中的重要作用，强调了α型CAs在决定植物生理和作物产量方面的重要性，为水稻高产品种的选育提供了遗传资源和新思路。
Plant growth and crop yield are essentially determined by photosynthesis when considering carbon dioxide (CO2) availability. CO2 diffusion inside a leaf is one of the factors that dictate the CO2 concentrations in chloroplasts. Carbonic anhydrases (CAs) are zinc-containing enzymes that interconvert CO2 and bicarbonate ions (HCO3−), which, consequently, affect CO2 diffusion and thus play a fundamental role in all photosynthetic organisms. Recently, the great progress in the research in this field has immensely contributed to our understanding of the function of the β-type CAs; however, the analysis of α-type CAs in plants is still in its infancy. In this study, we identified and characterized the OsαCA1 gene in rice via the analysis of OsαCAs expression in flag leaves and the subcellular localization of its encoding protein. OsαCA1 encodes an α-type CA, whose protein is located in chloroplasts with a high abundance in photosynthetic tissues, including flag leaves, mature leaves, and panicles. OsαCA1 deficiency caused a significant reduction in assimilation rate, biomass accumulation, and grain yield. The growth and photosynthetic defects of the OsαCA1 mutant were attributable to the restricted CO2 supply at the chloroplast carboxylation sites, which could be partially rescued by the application of an elevated concentration of CO2 but not that of HCO3−. Furthermore, we have provided evidence that OsαCA1 positively regulates water use efficiency (WUE) in rice. In summary, our results reveal that the function of OsαCA1 is integral to rice photosynthesis and yield potential, underscoring the importance of α-type CAs in determining plant physiology and crop yield and providing genetic resources and new ideas for breeding high-yielding rice varieties.
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影响因子：
64.8
作者：
Engineer, Cawas B.;Ghassemian, Majid;Anderson, Jeffrey C.;Peck, Scott C.;Hu, Honghong;Schroeder, Julian I.
通讯作者：
Schroeder, Julian I.
影响因子：
5.6
作者：
Hu L;Chen W;Yang W;Li X;Zhang C;Zhang X;Zheng L;Zhu X;Yin J;Qin P;Wang Y;Ma B;Li S;Yuan H;Tu B
通讯作者：
Tu B
影响因子：
11.4
作者：
Gong, Luping;Liao, Shenghao;Liang, Yun-Kuan
通讯作者：
Liang, Yun-Kuan
红豆杉的细胞壁特性影响叶绿体中层的二氧化碳传导性
影响因子：
9.4
作者：
Ellsworth, Patricia, V;Ellsworth, Patrick Z.;Cousins, Asaph B.
通讯作者：
Cousins, Asaph B.
影响因子：
7.4
作者：
Gillon, JS;Yakir, D
通讯作者：
Yakir, D