Identification of a cDNA clone for mouse apoprotein A-1 (apo A-1) and its use in characterization of apo A-1 mRNA expression in liver and small intestine.
Identification of a cDNA clone for mouse apoprotein A-1 (apo A-1) and its use in characterization of apo A-1 mRNA expression in liver and small intestine.
复制标题
小鼠脱辅基蛋白 A-1 (apo A-1) cDNA 克隆的鉴定及其在肝脏和小肠中 apo A-1 mRNA 表达表征中的应用。
DOI：
10.1073/pnas.80.6.1511
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Hastie,ND
中科院分区：
文献类型：
--
作者：
Miller,JC;Barth,RK;Shaw,PH;Elliott,RW;Hastie,ND
小鼠载脂蛋白A-1 （apo A-1）是血浆高密度脂蛋白的主要载脂蛋白。除了结构和生理证据外，小鼠血浆载脂蛋白a -1的遗传多态性已被用来证实该DNA序列与载脂蛋白a -1基因相对应。使用该克隆进行分子杂交研究表明，载脂蛋白A-1 mRNA的浓度在肝脏和小肠中相似，并且沿着小肠的整个长度是恒定的。我们提供的证据表明，相同的载脂蛋白A-1基因在肝脏和小肠中表达。载脂蛋白a - 1mrna也存在于胃和食道，浓度为小肠的10-15%，但在其他组织（如大肠、胰腺、心脏、肾脏、脾脏和脑）中检测不到。最后，我们发现高饱和脂肪和高胆固醇饮食对肝脏和小肠载脂蛋白a -1 mRNA水平有不同的影响。
A cDNA clone for mouse apoprotein A-1 (apo A-1), the major apoprotein of plasma high density lipoproteins, has been identified. In addition to structural and physiological evidence, a genetic polymorphism for mouse plasma apo A-1 has been used to confirm that this DNA sequence corresponds to the apo A-1 gene. Use of this clone in molecular hybridization studies has shown that the concentration of apo A-1 mRNA is similar in liver and small intestine and is constant along the entire length of the small intestine. We provide evidence that the same apo A-1 gene is expressed in both liver and small intestine. Apo A-1 mRNA is also present in the stomach and esophagus at 10-15% the concentration found in small intestine but is undetectable in other tissues (such as large intestine, pancreas, heart, kidney, spleen, and brain). Finally, we show that there is a differential effect of a diet high in saturated fat and cholesterol on apo A-1 mRNA levels in liver and small intestine.