IMMUNOASSAY USING ANTIGEN-ENZYME CONJUGATES
IMMUNOASSAY USING ANTIGEN-ENZYME CONJUGATES
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DOI：
10.1016/0014-5793(71)80319-8
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发表时间：
1971-01-01
期刊：
影响因子：
3.5
通讯作者：
SCHUURS, AHW
中科院分区：
文献类型：
--
作者：
VANWEEME.BK;SCHUURS, AHW
由Berson等人[1]发起的放射免疫分析的发展，使抗体和抗原的估计具有高度的敏感性。这在内分泌学中尤其如此，生物分析往往困难、耗时和昂贵。在这种类型的免疫分析中，抗原占据中心位置[2]，但其标记不必是放射性核素。Haimovich等人。已描述了用于抗体和抗原检测的蛋白质-噬菌体结合物[3，4]以及主要用于组织学目的的酶-抗体和酶-抗原结合物的制备[5，6]。我们将人绒毛膜促性腺激素(HCG)与辣根过氧化物酶(HRP)偶联，并用纯化的偶联物进行抗体和抗原的‘酶免疫分析’。
The development of the radio-immunoassay, initiated by Berson et al.[1], has permitted estimation of antibodies and antigens to a high degree of sensitivity. This is particularly true in endocrinology, where bioassays are often difficult, time consuming and expensive.In this type of immunoassay, the antigen occupies a central position [2] but its label, need not be a radionuclide. Haimovich et al. used protein-bacteriophage conjugates for the assay of antibodies and antigens [3, 4] and the preparation of enzyme-antibody and enzyme-antigen conjugates, mainly for histological purposes has been described [5, 6]. We have conjugated the antigen human chorionic gonadotrophin (HCG) to the enzyme horse radish peroxidase (HRP) and used purified conjugates for ‘enzyme-immunoassay’of antibody and antigen**.