A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae
A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae
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DOI：
10.1016/j.algal.2021.102191
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发表时间：
2021-01-11
影响因子：
5.1
通讯作者：
Ohama, Takeshi
中科院分区：
文献类型：
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作者：
Al-Azab, Ayat J. S.;Widyaningrum, Dwiyantari;Ohama, Takeshi
我们研究了树脂纳米颗粒(NPs)在不同非绿藻物种(例如，SAR和Hacrobia Clade中的物种)中诱导细胞死亡的效力，以及不同物种对NPs的敏感性差异。18种非绿藻与实验室制备的平均直径180 nm的聚氰基丙烯酸异丁酯树脂纳米粒(IBCA-NPs)共孵育24 h，暴露于100 mg L-1的IBCA-NPs中，诱导3种硅藻中的2种、所有3种隐藻、6种迪藻中的4种、4种甲藻中的3种和所有3种雷藻中的3种细胞死亡。然而，当L-1的暴露浓度增加到1g时，所有受试物种均可引起细胞死亡。对NP轨迹的观察表明，它们偶尔会与细胞碰撞并反弹离开，但不会附着在细胞表面。活性氧(ROS)清除剂N-乙酰-L-半胱氨酸显著延缓了细胞死亡诱导。这一发现有力地表明，ROS的产生是诱导细胞死亡的直接原因。缺乏覆盖结构的Raphitophyceae物种和不含球藻的Haptophyta菌株的细胞比具有覆盖结构的物种更敏感。此外，预包覆牛血清白蛋白或脱脂牛奶的IBCA-NPs严重降低了诱导细胞死亡的能力。我们提出了IBCA-NPs在多种藻类中诱导细胞死亡的假设机制，重点是细胞覆盖结构和ROS的产生。
We examined the potency of resin nanoparticles (NPs) to induce cell mortality in diverse non-green algal species (e.g., species in SAR and Hacrobia clades) and the difference in sensitivity to NPs among the species. In total, 18 non-green algal species were co-incubated with laboratory-prepared NPs (mean diameter, 180 nm) composed of poly (isobutylcyanoacrylate) resin NPs (iBCA-NPs).Exposure to 100 mg L-1 iBCA-NPs for 24 h induced cell death in two of three Bacillariophyceae species, all three Cryptophyceae species, four of six Dinophyta species, three of four Haptophyta strains, and all three Raphidophyceae species. However, exposure at an increased concentration of 1 g L-1 induced cell death in all the examined species. Observation of NP trails showed that they occasionally collided with the cells and bounced off but did not adhere to the cell surface. The reactive oxygen species (ROS) scavenger N-acetyl-L-cysteine substantially delayed cell death induction. This finding strongly suggests that ROS generation is a direct cause of induced cell death.The cells of Raphidophyceae species that lack covering structures and a Haptophyta strain bearing no coccoliths were more sensitive than species bearing covering structures. Moreover, iBCA-NPs pre-coated with bovine serum albumin or skim milk had severely reduced cell death-inducing potency. We propose a hypothetical mechanism of cell death induction by iBCA-NPs in a wide range of algal species, focusing on cell covering structures and ROS generation.